Bradykinin receptors in cultured rat dorsal root ganglion cells: influence of length of time in culture

Neuroscience
G Segond von BanchetB Heppelmann

Abstract

The endogenous nonapeptide bradykinin is a powerful substance which activates nociceptors, resulting in the sensation of pain in man. We used a newly developed non-radioactive method to detect bradykinin binding sites in isolated dorsal root ganglion cells with gold-labelled bradykinin. In a subpopulation of cells, gold-labelled bradykinin was bound in different quantities. The proportion of somata with bradykinin binding markedly depended on the length of time in culture. After 0.75 days, bradykinin was bound to 43% of somata. This proportion increased to 85% after 1.75 days and then decreased to 27% after 5.75 days. Bradykinin was bound to cells of all sizes, ranging from 40 to 2000 microns2 with a maximum of 200-300 microns2. In some cells, binding was also seen along the processes. No correlation was found between the soma size and the density of bradykinin binding. Blocking the bradykinin binding at the B1 receptor with (Des-Arg10)-Lys-bradykinin and at the B2 receptor with D-Arg(Hyp3-Thi5.8-D-Phe7)-bradykinin, respectively, revealed that in 0.75-day-old cultures no or only a very small amount of B1 receptors are present. In 1.75-day-old cultures, the marked increase in the proportion of cells with positive bradykinin bind...Continue Reading

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Citations

Aug 1, 1997·Current Opinion in Neurobiology·P Cesare, P McNaughton
Dec 20, 2002·International Immunopharmacology·Shigeyuki MatsuzakiMasataka Majima
Apr 3, 1999·British Journal of Pharmacology·S PooleS H Ferreira
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Jul 13, 2002·Annals of the New York Academy of Sciences·Hans-Georg SchaibleGisela Segond Von Banchet

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