PMID: 8599534Mar 1, 1996Paper

Bradyrhizobium japonicum possesses two discrete sets of electron transfer flavoprotein genes: fixA, fixB and etfS, etfL

Archives of Microbiology
M WeidenhauptH Hennecke

Abstract

A group of four co-regulated genes (fixA, fixB, fixC, fixX) essential for symbiotic nitrogen fixation has been described in several rhizobial species, including Bradyrhizobium japonicum. The complete nucleotide sequence of the B. japonicum fixA, fixB and fixC, genes is reported here. The derived amino acid sequences confirmed the previously noted sequence similarity between FixA and the beta-subunit and between FixB and the alpha-subunit of mammalian and Paracoccus denitrificans electron transfer flavoproteins (ETF). Since the classical role of ETF is in beta-oxidation of fatty acids, a process unrelated to nitrogen fixation, we rationalized that B. japonicum ought to contain bona fide etf genes in addition to the etf-like genes fixA and fixB. Therefore, we identified, cloned, sequenced, and transcriptionally analyzed the B. japonicum etfSL genes encoding the beta- and alpha-subunits of ETF. The etfSL genes, but not the fix genes, are transcribed in aerobically grown cells. An amino acid sequence comparison between all available ETFs and ETF-like proteins revealed the existence of two distinguishable subfamilies. Group I comprises housekeeping ETFs that link acyl-CoA dehydrogenase reactions with the respiratory chain, such as i...Continue Reading

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Citations

Oct 9, 2002·Environmental Microbiology·Achim QuaiserChrista Schleper
Dec 6, 2003·Genome Research·Chung-Yung ChenShih-Feng Tsai
Apr 12, 2011·Applied and Environmental Microbiology·Laure PratChristof Holliger
Feb 11, 2016·International Journal of Molecular Sciences·Dongxue XuHongsheng Yang
Aug 1, 2010·Environmental Microbiology·Jessica R SieberMichael J McInerney
Sep 10, 2020·Proceedings of the National Academy of Sciences of the United States of America·Rachel M WheatleyPhilip S Poole

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