PMID: 8464870Apr 1, 1993Paper

Bradyrhizobium japonicum rhizobitoxine genes and putative enzyme functions: expression requires a translational frameshift

Proceedings of the National Academy of Sciences of the United States of America
X RuanN K Peters

Abstract

Some strains of Bradyrhizobium japonicum produce rhizobitoxine, a phytotoxin that causes foliar chlorosis on susceptible host plants. We have previously obtained Tn5-induced rhizobitoxine null mutants of B. japonicum. DNA sequence analysis of the region surrounding two Tn5 insertions identifies two overlapping open reading frames. The first open reading frame (rtxA) predicts a 54-kDa protein for which the N-terminal 280 residues have sequence similarity to serine: pyruvate aminotransferase. The sequence homology to aminotransferase is consistent with the involvement of this gene in serinol production, a likely intermediate in rhizobitoxine biosynthesis. Previously, a mutant in this open reading frame was shown not to make serinol. The predicted amino acid sequence of the second open reading frame (rtxB) has similarity to yeast O-acetylhomoserine sulfhydrolase. This enzyme function is similar to that required for dihydrorhizobitoxine synthase. The DNA sequence shows that the rtxB open reading frame overlaps rtxA, suggesting that expression of rtxB requires a -1 translational frameshift. Protein expression experiments demonstrate production of an RtxAB fusion protein. The ability of the overlapping rtxA and rtxB sequences to prom...Continue Reading

References

Apr 1, 1992·Computer Applications in the Biosciences : CABIOS·D G HigginsR Fuchs
Aug 10, 1990·Cell·J F AtkinsR F Gesteland
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Nov 26, 1990·European Journal of Biochemistry·K NishiyamaA Ichiyama
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y Sekine, E Ohtsubo
Oct 24, 1986·Nucleic Acids Research·P KerjanY Surdin-Kerjan
Apr 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W R Pearson, D J Lipman
May 5, 1986·Journal of Molecular Biology·F W Studier, B A Moffatt
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·W J CraigenC T Caskey
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·G M WeinstockM Zweig
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski

❮ Previous
Next ❯

Citations

Jun 28, 2011·Applied Microbiology and Biotechnology·Björn Andreessen, Alexander Steinbüchel
Nov 23, 2011·Applied Microbiology and Biotechnology·Kazuhiro TakagiAkio Iwasaki
Oct 27, 2001·Applied and Environmental Microbiology·T YasutaK Minamisawa
Sep 13, 2011·AMB Express·Björn Andreeßen, Alexander Steinbüchel
Jun 20, 2015·SpringerPlus·María Dolores Molina-SánchezManuel Fernández-López
Jan 30, 1999·Applied and Environmental Microbiology·T YasutaK Minamisawa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.