BRAF is a therapeutic target in aggressive thyroid carcinoma

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Giuliana SalvatoreMassimo Santoro

Abstract

Oncogenic conversion of BRAF occurs in approximately 44% of papillary thyroid carcinomas and 24% of anaplastic thyroid carcinomas. In papillary thyroid carcinomas, this mutation is associated with an unfavorable clinicopathologic outcome. Our aim was to exploit BRAF as a potential therapeutic target for thyroid carcinoma. We used RNA interference to evaluate the effect of BRAF knockdown in the human anaplastic thyroid carcinoma cell lines FRO and ARO carrying the BRAF V600E (V600EBRAF) mutation. We also exploited the effect of BAY 43-9006 [N-(3-trifluoromethyl-4-chlorophenyl)-N'-(4-(2-methylcarbamoyl pyridin-4-yl)oxyphenyl)urea], a multikinase inhibitor able to inhibit RAF family kinases in a panel of six (V600E)BRAF-positive thyroid carcinoma cell lines and in nude mice bearing ARO cell xenografts. Statistical tests were two sided. Knockdown of BRAF by small inhibitory duplex RNA, but not control small inhibitory duplex RNA, inhibited the mitogen-activated protein kinase signaling cascade and the growth of ARO and FRO cells (P < 0.0001). These effects were mimicked by thyroid carcinoma cell treatment with BAY 43-9006 (IC50 = 0.5-1 micromol/L; P < 0.0001), whereas the compound had negligible effects in normal thyrocytes. ARO ce...Continue Reading

References

Nov 1, 1992·The Journal of Cell Biology·Y GavrieliS A Ben-Sasson
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·F CurcioH G Coon
Jan 1, 1993·The Journal of Clinical Investigation·J A FaginH P Koeffler
Jan 16, 1999·Seminars in Surgical Oncology·K B Ain
Sep 22, 2001·Endocrine-related Cancer·J F LyonsG Bollag
Dec 20, 2002·Current Opinion in Oncology·Janice L Pasieka
Feb 14, 2003·Lancet·Steven I Sherman
Apr 17, 2003·Journal of the National Cancer Institute·Yoram CohenDavid Sidransky
Sep 6, 2003·Cancer Cell·David A TuvesonMeenhard Herlyn
Sep 13, 2003·The Journal of Clinical Endocrinology and Metabolism·Hiroyuki NambaShunichi Yamashita
Mar 6, 2004·The Journal of Clinical Endocrinology and Metabolism·M XingD Sidransky
Mar 24, 2004·Cell·Paul T C WanUNKNOWN Cancer Genome Project
Jun 23, 2004·Oncogene·Maria KarasaridesRichard Marais
Nov 13, 2004·Nature·Jürgen SoutschekHans-Peter Vornlocher
Nov 19, 2004·Nature·Charles Sawyers
Dec 2, 2004·Nature Reviews. Cancer·Judith S Sebolt-Leopold, Roman Herrera
Dec 23, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Dirk StrumbergSiegfried Seeber
Mar 23, 2005·Cancer Research·Arati SharmaGavin P Robertson
Jun 11, 2005·Endocrine-related Cancer·M Xing
Sep 20, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Muralidhar BeeramEric K Rowinsky
Sep 22, 2005·The Journal of Clinical Endocrinology and Metabolism·Mingzhao XingPaul W Ladenson
Oct 14, 2005·Nature·Simon Frantz

❮ Previous
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Citations

Jul 5, 2013·Journal of Cancer Research and Clinical Oncology·Daniel ChanH Phillip Koeffler
May 4, 2012·Archives of Pharmacal Research·Do-Hee Kim, Taebo Sim
Jun 24, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Yuri E Nikiforov
Apr 6, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Steven I Sherman
May 18, 2010·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Monica ZerilliVito Rodolico
Nov 21, 2007·Nature Clinical Practice. Endocrinology & Metabolism·Ingrid G M Kolfschoten, Romano Regazzi
Oct 4, 2006·Nature Reviews. Drug Discovery·Scott WilhelmSusan Kelley
Aug 24, 2010·Oncogene·R M MelilloG Marone
Aug 29, 2007·Oncogene·C MichaloglouD S Peeper
Nov 2, 2012·Thyroid : Official Journal of the American Thyroid Association·Panayiotis SavvidesScot C Remick
Jan 1, 2013·Thyroid : Official Journal of the American Thyroid Association·Xinying LiEmad Kandil
Aug 31, 2006·Anti-cancer Drugs·Amalia MilanoFrancesco Caponigro
Aug 18, 2009·Pigment Cell & Melanoma Research·Leisl M PackerRichard Marais
Sep 3, 2010·American Journal of Physiology. Renal Physiology·Tamio YamaguchiDarren P Wallace
Nov 14, 2012·Therapeutic Advances in Endocrinology and Metabolism·David A Liebner, Manisha H Shah
Apr 18, 2012·Molecular Cancer·Michele MassiminoLivia Manzella
Mar 4, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Richard T KloosManisha H Shah
Mar 19, 2009·The Journal of Clinical Endocrinology and Metabolism·Rebecca E SchweppeBryan R Haugen
Dec 15, 2010·The Oncologist·Adrienne L MelckSally E Carty
Apr 22, 2011·Journal of Cancer·Rebecca L BrownEzra Ew Cohen
Nov 8, 2012·Future Oncology·Concetto RegalbutoVincenzo Pezzino
Feb 20, 2014·Virchows Archiv : an International Journal of Pathology·Renu K VirkManju L Prasad
Aug 10, 2011·Journal of Thyroid Research·Shannon R Bales, Inder J Chopra
Apr 13, 2007·Endocrine Journal·Atsushi KumagaiShunichi Yamashita
Mar 19, 2008·Acta Oncologica·Ramachandran VenkitaramanChristopher M Nutting
Oct 17, 2014·Expert Opinion on Pharmacotherapy·Jolanta Krajewska, Barbara Jarzab
Oct 13, 2009·Expert Review of Molecular Diagnostics·António E PintoJorge Soares
Jan 22, 2015·Expert Opinion on Pharmacotherapy·Jolanta KrajewskaBarbara Jarzab
Dec 20, 2007·Expert Review of Molecular Diagnostics·Marina N Nikiforova, Yuri E Nikiforov
Jan 19, 2010·Expert Opinion on Drug Safety·Benoit BlanchetFrançois Goldwasser
Feb 11, 2015·Expert Opinion on Drug Discovery·Peter T White, Mark S Cohen
Mar 17, 2010·Journal of the Chinese Medical Association : JCMA·Kam-Tsun Tang, Chen-Hsen Lee
Jan 11, 2014·Critical Reviews in Oncology/hematology·Ozgur Omur, Yusuf Baran
Dec 29, 2015·IUBMB Life·Sithul Hima, Sreeharshan Sreeja
Apr 23, 2013·The Journal of Surgical Research·Emad KandilAsim B Abdel-Mageed
Jan 16, 2016·Annual Review of Medicine·Warren Fiskus, Nicholas Mitsiades
Feb 3, 2007·The Journal of Investigative Dermatology·Giovanni PontiStefania Seidenari
May 27, 2008·Endocrinology and Metabolism Clinics of North America·Thomas J Giordano
May 27, 2008·Endocrinology and Metabolism Clinics of North America·Manuel Sobrinho-SimõesPaula Soares
Mar 14, 2008·Trends in Endocrinology and Metabolism : TEM·Efisio PuxedduDiego Russo

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