BRAF, KIT and NRAS mutations and expression of c-KIT, phosphorylated extracellular signal-regulated kinase and phosphorylated AKT in Japanese melanoma patients

The Journal of Dermatology
Satomi OyamaHidehisa Saeki

Abstract

To clarify the status of gene mutation and activation of growth signal in melanoma of Japanese patients in vivo, we analyzed the mutation of BRAF exon 15, NRAS exon 2, and KIT exons 9, 11, 13, 17 and 18 in melanoma cells obtained by laser capture microdissection, and performed direct sequencing in 20 cases of acral lentiginous melanoma (ALM) and 17 cases of superficial spreading melanoma (SSM). In the study of the mutation of BRAF, pyrosequencing was also done. To examine the cell proliferation signaling, immunohistochemistry for phosphorylated extracellular signal-regulated kinase (pERK), phosphorylated AKT (phosphorylated AKT) and c-KIT was done. The mutation of BRAF p.V600E was detected in 13 cases of ALM (65.0%) and 12 cases of SSM (70.6%). No NRAS mutation was found in all cases. The mutation in exons 9, 11, and 18 of KIT was detected in nine cases. The mutation of BRAF and KIT showed no correlation with clinical stage, lymph node metastasis, tumor thickness, ulceration and histology. pERK and pAKT was observed in small population of melanoma cells and there was no correlation with gene mutation. Our results indicate that the mutations of BRAF and KIT exist in Japanese melanoma patients, however, the cell growth signaling ...Continue Reading

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Citations

Jan 28, 2016·The Journal of Dermatology·Masutaka Furue, Takafumi Kadono
Jan 30, 2016·Expert Review of Molecular Diagnostics·Roberta CastiglioneReinhard Büttner
Jan 18, 2017·Journal of Cancer Research and Clinical Oncology·Hui LiuWeicheng Xue
May 5, 2016·Advances in Anatomic Pathology·Brendon MitchellMeera Mahalingam
Aug 9, 2019·The American Journal of Dermatopathology·Andréia Neves Comodo-NavarroGilles Landman
Aug 11, 2020·BioMed Research International·Luz D Gutiérrez-CastañedaJose D Tovar-Parra

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