BRAF mutation testing with a rapid, fully integrated molecular diagnostics system

Oncotarget
Filip JankuRazelle Kurzrock

Abstract

Fast and accurate diagnostic systems are needed for further implementation of precision therapy of BRAF-mutant and other cancers. The novel IdyllaTMBRAF Mutation Test has high sensitivity and shorter turnaround times compared to other methods. We used Idylla to detect BRAF V600 mutations in archived formalin-fixed paraffin-embedded (FFPE) tumor samples and compared these results with those obtained using the cobas 4800 BRAF V600 Mutation Test or MiSeq deep sequencing system and with those obtained by a Clinical Laboratory Improvement Amendments (CLIA)-certified laboratory employing polymerase chain reaction-based sequencing, mass spectrometric detection, or next-generation sequencing. In one set of 60 FFPE tumor samples (15 with BRAF mutations per Idylla), the Idylla and cobas results had an agreement of 97%. Idylla detected BRAF V600 mutations in two additional samples. The Idylla and MiSeq results had 100% concordance. In a separate set of 100 FFPE tumor samples (64 with BRAF mutation per Idylla), the Idylla and CLIA-certified laboratory results demonstrated an agreement of 96% even though the tests were not performed simultaneously and different FFPE blocks had to be used for 9 cases. The IdyllaTMBRAF Mutation Test produced ...Continue Reading

References

Apr 5, 2001·The New England Journal of Medicine·B J DrukerC L Sawyers
Sep 8, 2010·The New England Journal of Medicine·Keith T FlahertyPaul B Chapman
Oct 29, 2010·The New England Journal of Medicine·Eunice L KwakA John Iafrate
Jun 7, 2011·The New England Journal of Medicine·Paul B ChapmanUNKNOWN BRIM-3 Study Group
Oct 13, 2012·Pathology, Research and Practice·Soya S SamJoel A Lefferts
Dec 15, 2012·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Oliver KleinGeorgina V Long
Jun 5, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Solange PetersStefan Zimmermann
Jun 5, 2013·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Javier MunozRazelle Kurzrock
Jan 21, 2014·Cell Reports·Filip JankuRazelle Kurzrock

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Citations

Nov 6, 2015·Journal of Clinical Pathology·Richard CollingElizabeth Soilleux
Apr 29, 2016·Journal of Clinical Pathology·Dario de BiaseGiancarlo Troncone
Jun 16, 2018·Journal of Clinical Pathology·Arnaud Uguen, Giancarlo Troncone
Oct 11, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Pawel SadleckiMalgorzata Walentowicz-Sadlecka
May 22, 2019·Journal of Clinical Pathology·Caterina De LucaGiancarlo Troncone
Dec 20, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Seyed PairawanFunda Meric-Bernstam
May 11, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Pawel SadleckiMalgorzata Walentowicz-Sadlecka
Dec 14, 2019·The American Journal of Dermatopathology·Danielle R PettyStacey S O'Neill

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Methods Mentioned

BETA
PCR
dissection
biopsies
CLIA

Software Mentioned

MiSeq
Idylla TM
GraphPad
Idylla

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