Brahma-related gene 1 inhibits proliferation and migration of human aortic smooth muscle cells by directly up-regulating Ras-related associated with diabetes in the pathophysiologic processes of aortic dissection

The Journal of Thoracic and Cardiovascular Surgery
Wei-Lin LiaoZhiyun Xu

Abstract

To elucidate the mechanisms of Brahma-related gene 1 (Brg1) involvement in the pathophysiologic processes of aortic dissection. Seventeen dissecting, 4 dilated, and 10 healthy human aorta samples were collected. Expression of Brg1 in the medium of aorta was evaluated by quantitative real-time polymerase chain reaction, Western blot, and immunohistochemical staining, respectively. The regulation effect of Brg1 on proliferation and migration of human aortic smooth muscle cells (HASMCs) was analyzed in 3 ways: using cell counting, a migration chamber, and a wound scratch assay. A polymerase chain reaction array was used for screening potential target genes of Brg1. A chromatin immunoprecipitation assay was adopted for direct deoxyribonucleic acid-protein binding detection. Expression levels of Brg1 were increased in aortic dissection and aortic dilation patients. In vitro results indicated that overexpression of Brg1 inhibited proliferation and migration of HASMCs. The candidate proliferation- and migration-related Brg1 target gene found was Ras-related associated with diabetes (RRAD), expression levels of which were enhanced in dissecting aortic specimens. The direct regulation effect of Brg1 on RRAD was verified by chromatin imm...Continue Reading

References

Aug 14, 1999·Science·F G Giancotti, E Ruoslahti
Dec 17, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Qi Jun ZhangMartin Bennett
Dec 16, 2003·Molecular and Cellular Biology·Kristin B HendricksEmma Lees
Dec 30, 2006·Annals of Surgical Oncology·Makoto SuzukiAdi F Gazdar
Mar 17, 2007·Circulation Research·William T Gerthoffer
Jun 2, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Vyacheslav A KorshunovBradford C Berk
Jun 30, 2007·The Journal of Biological Chemistry·Min ZhangB Paul Herring
Dec 7, 2007·Circulation·Lin ChangY Eugene Chen
Jun 12, 2008·Annual Review of Genomics and Human Genetics·Dianna M MilewiczHariyadarshi Pannu
Jul 8, 2008·Lancet·Jonathan Golledge, Kim A Eagle
Apr 4, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Jiliang ZhouB Paul Herring
Jan 30, 2010·Nature·Lena Ho, Gerald R Crabtree
Dec 22, 2010·Nature Reviews. Cardiology·Scott A LeMaire, Ludivine Russell
Jan 12, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Bo-Yuan HsiaoTeh-Ying Chou
Jan 26, 2011·Proceedings of the National Academy of Sciences of the United States of America·Courtney T GriffinTerry Magnuson
Mar 9, 2011·Cardiovascular Research·Ji ZhangXiaojun Zhu
Mar 1, 2012·Journal of Cancer Research and Clinical Oncology·Jin BaiJun-Nian Zheng
Apr 3, 2012·Cardiovascular Research·Patrick LacolleyJean-Baptiste Michel
Jul 31, 2012·The Journal of Thoracic and Cardiovascular Surgery·Bartosz RylskiMatthias Siepe
Aug 24, 2013·The Journal of Thoracic and Cardiovascular Surgery·Mi ZhangZhiyun Xu
May 31, 2014·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Shuwei WangZhiyun Xu
Oct 12, 2014·BMC Cardiovascular Disorders·Yang YuanShengdong Huang

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Citations

Sep 24, 2015·The Journal of Thoracic and Cardiovascular Surgery·Alden H Harken
Oct 26, 2018·Circulation Research·Weiqi ZhangGuang-Hui Liu
Dec 7, 2021·Frontiers in Cardiovascular Medicine·Qian WangJianbing Zhu

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