PMID: 7541321Apr 18, 1995Paper

Brain-derived neurotrophic factor (BDNF) can prevent apoptosis of rat cerebellar granule neurons in culture

Brain Research. Developmental Brain Research
T KuboH Hatanaka

Abstract

Cerebellar granule neurons obtained from 9-day-old rats were grown in vitro for 4 days in high K+ (26 mM) medium. The culture medium was then replaced with that containing low K+ (5 mM) which caused a large number of granule neurons to die. The death of granule neurons has been characterized as apoptosis. In this study, we investigated the effects of various neurotrophins on neuronal survival using the above system. We found that brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5 (NT-4/5) but not nerve growth factor (NGF) can protect these neurons from apoptosis in low K+. Neurotrophin-3 (NT-3) had a small effect on neuronal survival as reported. To determine whether the granule neurons respond directly to BDNF, we analyzed the induction of the Fos protein in these neurons. Individual cells that synthesize Fos protein after exposure to neurotrophin can be recognized using antibodies to Fos. Immunocytochemical staining of the cultures demonstrated that a relatively large number of cerebellar granule neurons showed immunoreactivity in response to BDNF, but few of them were immunoreactive in the absence of BDNF or in the presence of NGF. Our results suggested that BDNF has a direct effect on mature cerebellar granule ne...Continue Reading

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Citations

Sep 25, 2002·Journal of Neuroscience Research·Jacques Borg, Jacqueline London
May 10, 2011·Cellular and Molecular Neurobiology·Hisatsugu KoshimizuHiroshi Hatanaka
Aug 1, 1996·Journal of the Neurological Sciences·A M GormanT Cotter
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Nov 22, 1996·Brain Research. Developmental Brain Research·T NonomuraH Hatanaka
Nov 22, 1996·Brain Research. Developmental Brain Research·T OkaH Hatanaka
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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis