Brain-derived neurotrophic factor increases survival and differentiated functions of rat septal cholinergic neurons in culture

Neuron
R F AldersonR M Lindsay

Abstract

Brain-derived neurotrophic factor (BDNF) was found to promote the survival of E17 rat embryo septal cholinergic neurons in culture, as assessed by a histochemical stain for acetylcholinesterase (AChE). A 2.4-fold increase in neuronal survival was achieved with 10 ng/ml BDNF. After initial deprivation of growth factor for 7 days, BDNF failed to bring about this increase, strongly suggesting that BDNF promotes cell survival and not just induction of AChE. BDNF was also found to increase the levels of cholinergic enzymes; choline acetyltransferase (ChAT) and AChE activities were increased by approximately 2-fold in the presence of 50 ng/ml BDNF. BDNF produced a 3-fold increase in the number of cells bearing the NGF receptor, as detected by the monoclonal antibody IgG-192. Although NGF had no additive effect with BDNF in terms of neuronal survival, suggesting that both act on a similar neuronal population, the combination of both produced an additive response, approximately a 6-fold increase, in ChAT activity.

References

May 24, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·X Liu, R Jaenisch
Jul 9, 1999·Journal of Neuroscience Research·A NittaS Furukawa
Mar 25, 1994·Biotechnology and Bioengineering·R Bellamkonda, P Aebischer
Feb 1, 1993·Journal of Neuroscience Research·W HamelK Nikolics
Apr 15, 1993·Journal of Neuroscience Research·E EscandónK Nikolics
Apr 29, 1998·Molecular Neurobiology·K Fukunaga, E Miyamoto
Aug 1, 1994·Molecular Neurobiology·D P Kuffler
Jun 20, 2006·European Archives of Psychiatry and Clinical Neuroscience·Chie YasutakeHiroshi Yoneda
Aug 10, 2004·Cell and Tissue Research·Kerstin Krieglstein
Jan 19, 2012·Cellular and Molecular Neurobiology·Jan BakosZuzana Bacova
Dec 30, 2011·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Shiou-Lan ChenRu-Band Lu
Jan 22, 2013·Journal of Molecular Neuroscience : MN·Jan BakosZuzana Bacova
Jan 1, 1994·Life Sciences·A Kupsch, W H Oertel
Jan 1, 1994·Life Sciences·R Hellweg, M Jockers-Scherübl
Jan 1, 1994·Neuroscience and Biobehavioral Reviews·L M ManessJ E Zadina
Nov 20, 1992·Brain Research. Developmental Brain Research·G W HuntleyP J Isackson
Dec 18, 1992·Brain Research. Developmental Brain Research·D NonnerJ N Barrett
Jul 16, 1993·Brain Research. Developmental Brain Research·M DownenB H Wainer
Mar 19, 1993·Brain Research. Developmental Brain Research·T RingstedtH Persson
Nov 18, 1994·Brain Research. Developmental Brain Research·R LinkeM Frotscher
Nov 1, 1992·Trends in Pharmacological Sciences·C M Gall
May 1, 1991·Trends in Neurosciences·H Thoenen
Aug 1, 1991·Trends in Neurosciences·E Cattaneo, R McKay

Citations

Mar 23, 1990·Science·P C MaisonpierreG D Yancopoulos
Jun 1, 1989·Neuron·Y A Barde
Mar 1, 1988·The Journal of Comparative Neurology·K Semba, H C Fibiger
Feb 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S R WhittemoreH Persson
Oct 17, 1986·Science·T H LargeL F Reichardt
Sep 15, 1974·The Journal of Comparative Neurology·E S Creps
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S Korsching, H Thoenen
Jan 1, 1990·The European Journal of Neuroscience·R M LindsayD. Lindholm
Jan 1, 1989·The European Journal of Neuroscience·Solon ThanosJens Vanselow

Related Concepts

Embryo
Monoclonal Antibodies
Enzymes, antithrombotic
Entire Embryo
Hormone Receptors, Cell Surface
Ache
Neurons
Brain
Recombinant Brain-Derived Growth Factors
Brain-Derived Neurotrophic Factor Measurement

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.