PMID: 1946410Nov 1, 1991

Brain-derived neurotrophic factor: subcellular compartmentalization and interneuronal transfer as visualized with anti-peptide antibodies

Proceedings of the National Academy of Sciences of the United States of America
C WetmoreL Olson

Abstract

The recent cloning of a second member of the nerve growth factor family, brain-derived neurotrophic factor (BDNF), has prompted investigation into the cells that express this factor's mRNA and protein. In the present study, antibodies raised against unique peptide sequences within the porcine BDNF protein detect BDNF-like immunoreactivity in neurons in rat hippocampal and cortical areas consistent with the distribution of BDNF mRNA as detected with in situ hybridization. Within these neurons, BDNF-like immunoreactivity was observed in the cytoplasm, dendrites, and nuclei. In addition, BDNF immunoreactivity was observed in the cytoplasm of cholinergic neurons that do not express detectable levels of BDNF mRNA. Thus, anti-peptide antibodies can be used to detect this neurotrophic factor protein in cytoplasmic sites of synthesis and in areas of probable action. We propose that one form of the BDNF protein enters the nucleus and may directly influence transcription, while another fraction of the protein is transported out of the synthesizing cell and can be detected, after retrograde axonal transport, in cytoplasmic granules in the perikarya of cholinergic neurons. These basal forebrain cholinergic neurons project to regions enrich...Continue Reading

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Related Concepts

Immunofluorescence Assay
Immunoreactivity
August Rats
Neurons
Nerve Growth Factor
Recombinant Brain-Derived Growth Factors
Brain-Derived Neurotrophic Factor Measurement
Bdnf
Neurotrophic factor
Cell Nucleus

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