Brain distribution of nitric oxide synthase in neuronal or endothelial nitric oxide synthase mutant mice using [3H]L-NG-nitro-arginine autoradiography

Neuroscience
H HaraM A Moskowitz

Abstract

The regional distribution of nitric oxide synthase in the central nervous system was assessed by quantitative autoradiography using [3H]L-NG-nitro-arginine binding in wild-type mice (SV-129 and C57black/6) and in mice lacking expression of the neuronal (type 1) and endothelial (type 3) nitric oxide synthase gene. The distribution of nitric oxide synthase binding sites in wild-type mice was similar to that described for rat brain by nicotinamide adenine dinucleotide phosphate-diaphorase staining and immunohistochemistry, and as determined by quantitative autoradiography. In the wild-type mice, the densest labelling was observed in the granular layer of the olfactory bulb, tenia tecta, rhinal fissure, amygdaloid complex and molecular layer of cerebellum. The islands of Calleja, the hippocampal CA1 and CA3 subfields, dentate gyrus, cortical layers I-II, the superficial gray layer of superior colliculus and the granule layer of cerebellum displayed intermediate binding. Cortical layers III-VI, the striatum and the thalamus were only weakly labelled. Binding was saturable and of high affinity, and was displaced by 7-nitroindazole (100 microM), a potent and selective inhibitor of type 1 nitric oxide synthase, and by unlabelled L-NG-n...Continue Reading

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Citations

Oct 30, 2003·Viral Immunology·Fredrik AronssonKrister Kristensson
May 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H HaraM A Moskowitz
Nov 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dean A LeMichael A Moskowitz
Aug 5, 2000·Hormones and Behavior·S C GammieR J Nelson
May 20, 2004·European Urology·William J Martin, D Euan MacIntyre
Oct 4, 2003·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·D N AtochinP L Huang
Aug 3, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Bojana StefanovicAfonso C Silva
Oct 24, 2007·Brain Research Reviews·Arthur Giraldi-GuimarãesRosalia Mendez-Otero
Jul 12, 2005·Schizophrenia Research·Hans-Gert BernsteinGerburg Keilhoff
Jan 20, 2010·Pharmaceuticals·Gregers Wegener, Vallo Volke
Jul 12, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Koji SakaiKenji Sunagawa
Oct 7, 2010·Journal of Nutritional Science and Vitaminology·Atsumi Hayashi, Koichi Kimoto
Mar 5, 1998·The American Journal of Physiology·W MengM A Moskowitz
Dec 9, 1998·The American Journal of Physiology·I DumontS Chemtob
Apr 19, 2021·Behavioural Brain Research·Julio César Morales-MedinaGonzalo Flores
Aug 14, 2021·Journal of Chemical Neuroanatomy·Sonia Irais González-CanoGonzalo Flores

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