Brain heating induced by near-infrared lasers during multiphoton microscopy

Journal of Neurophysiology
Kaspar Podgorski, Gayathri Ranganathan


Two-photon imaging and optogenetic stimulation rely on high illumination powers, particularly for state-of-the-art applications that target deeper structures, achieve faster measurements, or probe larger brain areas. However, little information is available on heating and resulting damage induced by high-power illumination in the brain. In the current study we used thermocouple probes and quantum dot nanothermometers to measure temperature changes induced by two-photon microscopy in the neocortex of awake and anaesthetized mice. We characterized heating as a function of wavelength, exposure time, and distance from the center of illumination. Although total power is highest near the surface of the brain, heating was most severe hundreds of micrometers below the focal plane, due to heat dissipation through the cranial window. Continuous illumination of a 1-mm(2) area produced a peak temperature increase of ∼1.8°C/100 mW. Continuous illumination with powers above 250 mW induced lasting damage, detected with immunohistochemistry against Iba1, glial fibrillary acidic protein, heat shock proteins, and activated caspase-3. Higher powers were usable in experiments with limited duty ratios, suggesting an approach to mitigate damage in h...Continue Reading


Jul 1, 1991·Journal of Neuroscience Methods·J C Magee, G G Schofield
Mar 5, 1971·Science·R L Fork
Jan 1, 1997·Neuroscience and Biobehavioral Reviews·F C BaroneR F White
Mar 29, 2000·Biophysical Journal·G H Patterson, D W Piston
Nov 4, 2000·Journal of Biomedical Science·J W ChenG Campbell
Mar 22, 2001·Biophysical Journal·A Hopt, E Neher
May 2, 2002·Journal of Neurobiology·Hajime HiraseWeijian Yang
Jan 14, 2003·Biophysical Journal·Giuseppe ChiricoAlberto Diaspro
May 15, 2003·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·James R Lepock
May 15, 2003·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·M W DewhirstP J Hoopes
Nov 19, 2003·Biochemical and Biophysical Research Communications·Hiroyuki KawanoKatsumi Midorikawa
Aug 1, 1949·The Journal of Physiology·A L HODGKIN, B KATZ
May 14, 2005·Brain Research. Brain Research Reviews·Eugene A Kiyatkin
Jul 15, 2005·Best Practice & Research. Clinical Anaesthesiology·Robert D SandersMervyn Maze
Dec 21, 2006·Nature Methods·Gerald DonnertS W Hell
Feb 16, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Koji ShibasakiMakoto Tominaga
Apr 13, 2007·European Journal of Applied Physiology·Eugene A Kiyatkin
May 29, 2007·Biophysical Journal·Jonathon WellsE Duco Jansen
Aug 31, 2007·Nano Letters·Sha LiHaw Yang
Dec 18, 2007·Optics Letters·A Schönle, S W Hell
Dec 19, 2007·Optics Letters·J BewersdorfS W Hell
Feb 17, 2009·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·W C Dewey
Aug 20, 2007·Optics Express·Karsten BahlmannKarl Bellve
Jan 11, 2011·Nature Methods·Adrian ChengCarlos Portera-Cailliau
May 14, 2011·Small·Laura M MaestroJosé García Solé
Nov 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dmitriy AronovMichale S Fee
Feb 23, 2012·Nature Reviews. Neuroscience·Midori A Yenari, Hyung Soo Han
Mar 15, 2012·Nature Communications·Mikhail G ShapiroFrancisco Bezanilla
Sep 14, 2012·Journal of Neurophysiology·Abigail S Kalmbach, Jack Waters
Jul 13, 2013·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Efterpi PavlidouChristos Panteliadis
Jul 23, 2013·Nature·Tsai-Wen ChenDouglas S Kim
Aug 2, 2013·Nature·G KucskoM D Lukin
Jun 24, 2014·Nature Methods·Daniel R HochbaumAdam E Cohen
Jul 9, 2014·The Journal of Physical Chemistry. B·Mikhail DrobizhevAleksander Rebane
Sep 25, 2014·PloS One·Hod DanaKarel Svoboda
Nov 18, 2014·Nature Neuroscience·Jérôme LecoqMark J Schnitzer
May 15, 2015·Journal of Neurophysiology·Michael J ShireyPatrick J Drew
Jul 15, 2015·Cell Reports·Joseph M StujenskeJoshua A Gordon
Oct 16, 2015·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Valentina EmilianiMichael Häusser


Nov 1, 2016·Nature Methods·Robert PrevedelAlipasha Vaziri
Mar 21, 2017·Nature Methods·Alexander SongDavid W Tank
Apr 1, 2017·Nature Methods·Weijian Yang, Weijian Yang
Nov 14, 2017·Journal of Neural Engineering·Renaud SchuckSimon R Schultz
May 2, 2018·Nature Neuroscience·Alan R MardinlyHillel Adesnik
May 2, 2018·Annual Review of Neuroscience·Siegfried Weisenburger, Alipasha Vaziri
Aug 30, 2016·Nature Neuroscience·Na JiSpencer L Smith
Aug 30, 2016·Nature Neuroscience·Kenneth D HarrisSpencer L Smith
Oct 11, 2019·Nature·Kiryl D PiatkevichXue Han
May 18, 2018·Nature Communications·Jochen MeyerStelios Smirnakis
Apr 3, 2019·Nature Chemical Biology·Younghee OhMichael Z Lin
Jun 19, 2019·Nature Neuroscience·Scott F OwenAnatol C Kreitzer
Sep 26, 2019·Frontiers in Synaptic Neuroscience·Aundrea F BartleyLynn E Dobrunz
Oct 30, 2019·Nature Methods·Tong ZhangMark J Schnitzer
Sep 11, 2020·Optics Express·M Hossein EybposhNicolas C Pégard
Nov 7, 2019·Optics Express·Sanaz SadeghYeshaiahu Fainman
Aug 1, 2019·Nature Methods·Abbas KazemipourKaspar Podgorski
Feb 11, 2020·Frontiers in Cellular Neuroscience·Aniruddha DasHod Dana
Apr 3, 2020·Nature·Oleg I RumyantsevMark J Schnitzer
May 27, 2020·Nature Methods·Manuel Alexander MohrKaspar Podgorski
Jul 19, 2017·Biomedical Optics Express·Fabian F VoigtUrsula Keller
May 21, 2019·Nano Research·Jackson Travis Del Bonis-O'DonnellMarkita P Landry
Jul 10, 2019·Annual Review of Neuroscience·Elizabeth Mc HillmanHang Yu
May 29, 2018·Nature Neuroscience·Adam E Cohen, Samouil L Farhi
Nov 28, 2020·Nature Communications·Jiang Lan FanNa Ji
Dec 31, 2020·PloS One·Merrilee Thomas, Thomas E Hughes
Feb 9, 2021·Biosensors & Bioelectronics·Tucker StuartPhilipp Gutruf
Nov 6, 2020·Biomedical Optics Express·Vincent MaioliWilly Supatto
Feb 20, 2021·Nature Neuroscience·David A HartmannAndy Y Shih
Mar 10, 2021·Nature Biomedical Engineering·Sang Min WonJohn A Rogers
Apr 12, 2018·Current Opinion in Neurobiology·Elizabeth Mc HillmanPubudu T Galwaduge
Apr 17, 2018·Current Opinion in Neurobiology·Weijian Yang, Rafael Yuste
Feb 20, 2021·Journal of Neural Engineering·Le Cai, Philipp Gutruf
Feb 28, 2020·Current Opinion in Biomedical Engineering·Mitchell Clough, Jerry L Chen

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