PMID: 9188592Jul 1, 1997Paper

Brain-infiltrating cytolytic T lymphocytes specific for Theiler's virus recognize H2Db molecules complexed with a viral VP2 peptide lacking a consensus anchor residue

Journal of Virology
N D BorsonP J Wettstein

Abstract

Mice expressing the H2b haplotype are resistant to infection with Theiler's murine encephalomyelitis virus (TMEV), which causes chronic demyelination in susceptible mice. The prominent cytolytic T-lymphocyte (CTL) response to the VP2 antigen encoded by TMEV led us to the identification of a class I-binding peptide derived from the VP2 antigen. Escherichia coli transformants overexpressing a series of 11 overlapping VP2 protein fragments were subjected to lysis and alkali digestion, and the resultant peptide pools were tested for their abilities to sensitize RMA-S targets for lysis by CTLs. The source of effector CD8+ T cells for the assays was either freshly harvested central nervous system-infiltrating lymphocytes (CNS-IL) or CNS-IL-derived VP2-specific CTL clones and lines. A 10-residue peptide at VP2 positions 121 to 130 (VP2(121-130)) (FHAGSLLVFM) was identified that sensitized targets for lysis and formed stable complexes with H2Db class I molecules. The VP2(121-130) peptide sensitized target cells for lysis by freshly harvested CNS-IL CTLs at femtomolar concentrations. Despite its relative high level of biological activity, the VP2(121-130) peptide is distinguished from other Db-binding peptides by its lack of an asparagi...Continue Reading

References

Jul 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·P J WettsteinJ A Frelinger
Feb 1, 1990·The Journal of Experimental Medicine·F R Carbone, M J Bevan
Aug 2, 1990·Nature·H G LjunggrenK Kärre
Mar 1, 1986·The Journal of Experimental Medicine·M RodriguezC S David
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·P B ChallonerM Chang
Apr 1, 1993·Seminars in Immunology·K Falk, O Rötzschke
Jan 1, 1995·Immunogenetics·H G RammenseeS Stevanoviíc
Jan 1, 1993·Journal of Virology·L FietteC P Rossi
Mar 1, 1993·Journal of Neuropathology and Experimental Neurology·I Allen, B Brankin
Jan 1, 1993·Annual Review of Immunology·H G RammenseeO Rötzschke
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·M Kovacsovics-BankowskiK L Rock

❮ Previous
Next ❯

Citations

May 24, 2000·Journal of Virology·A Azoulay-CaylaJ F Bureau
Nov 7, 2009·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Ikuo Tsunoda, Robert S Fujinami
May 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aaron J JohnsonLarry R Pease
Sep 26, 2008·Journal of Virology·Zhiguo LiangHoward L Lipton
Sep 13, 2005·Annual Review of Microbiology·Michel BrahicThomas Michiels
Oct 19, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A J JohnsonL R Pease
Aug 2, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zachariah P TritzAaron J Johnson
Mar 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·K M DrescherM Rodriguez
Mar 4, 2000·International Immunology·K M DrescherM Rodriguez
Aug 18, 2010·Cellular and Molecular Life Sciences : CMLS·Tanja ScheiklRoland S Liblau
Jan 24, 2019·International Journal of Molecular Sciences·Ingo GerhauserAndreas Beineke
Oct 22, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M Brahic, J F Bureau

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.