Brain malformations in ovine fetuses associated with the cytopathogenic biotype of bovine viral-diarrhoea virus

Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B
A D GruberG Trautwein

Abstract

A total of six ewes were intravenously inoculated at between 65 and 68 days of gestation with the Indiana strain of bovine viral diarrhoea virus (BVDV), containing both non-cytopathogenic (ncp) and cytopathogenic (cp) biotypes. Eight transplacentally infected fetuses were sequentially removed from the infected ewes and were found to have inflammatory lesions and malformations of the brain. BVDV RNA was isolated from formalin-fixed, paraffin-embedded brain tissue sections and detected by nested polymerase chain reaction after reverse transcription. The two biotypes of BVDV were distinguished by the fact that a sequence insertion in the RNA of the cp biotype of the inoculum results in larger amplicons. Only RNA from cp BVDV was detected in three of the brains removed up to 14 days post-inoculation (p.i.), and no BVDV RNA was detected after more than 14 days p.i. These findings suggest that, in critical phases of development, cp BVDV may transplacentally infect the ovine fetal brain and cause malformations.

References

Jan 1, 1992·Advances in Virus Research·V Moennig, P G Plagemann
Oct 12, 1989·Nature·G MeyersH J Thiel
Dec 1, 1994·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·A D GruberG Trautwein
Mar 1, 1993·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·B LiessV Moennig
Jun 1, 1994·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M Hewicker-TrautweinG Trautwein

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Citations

Nov 1, 1995·Zentralblatt für Veterinärmedizin. Reihe B. Journal of veterinary medicine. Series B·M Hewicker-TrautweinB Liess
Nov 4, 2009·Animal Health Research Reviews·Thomas Passler, Paul H Walz
Mar 16, 2004·Veterinary Pathology·U Blas-MachadoS L Caseltine

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