Nov 29, 2016

Brain microbiota disruption within inflammatory demyelinating lesions in multiple sclerosis

Scientific Reports
William G BrantonChristopher Power

Abstract

Microbial communities reside in healthy tissues but are often disrupted during disease. Bacterial genomes and proteins are detected in brains from humans, nonhuman primates, rodents and other species in the absence of neurological disease. We investigated the composition and abundance of microbiota in frozen and fixed autopsied brain samples from patients with multiple sclerosis (MS) and age- and sex-matched nonMS patients as controls, using neuropathological, molecular and bioinformatics tools. 16s rRNA sequencing revealed Proteobacteria to be the dominant phylum with restricted diversity in cerebral white matter (WM) from MS compared to nonMS patients. Both clinical groups displayed 1,200-1,400 bacterial genomes/cm3 and low bacterial rRNA:rDNA ratios in WM. RNAseq analyses showed a predominance of Proteobacteria in progressive MS patients' WM, associated with increased inflammatory gene expression, relative to a broader range of bacterial phyla in relapsing-remitting MS patients' WM. Although bacterial peptidoglycan (PGN) and RNA polymerase beta subunit immunoreactivities were observed in all patients, PGN immunodetection was correlated with demyelination and neuroinflammation in MS brains. Principal component analysis reveal...Continue Reading

  • References40
  • Citations36

References

  • References40
  • Citations36

Citations

Mentioned in this Paper

Immunoreactivity
Nonhuman primate
Genome
Autopsy
Brain
Entire Cerebral White Matter
DNA, Ribosomal
Bio-Informatics
Ribosomal RNA
Organ

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