PMID: 2501450Aug 1, 1989Paper

Brain microvessels produce 12-hydroxyeicosatetraenoic acid

Journal of Neurochemistry
S A MooreM N Hart

Abstract

Cerebral microvessels isolated from perfused, adult murine brain produce a compound with the chromatographic properties of a monohydroxyeicosatetraenoic acid when incubated with arachidonic acid or stimulated with calcium ionophore A23187. The formation of this arachidonic acid metabolite is not reduced in the presence of the cyclooxygenase inhibitor ibuprofen, but it is abolished by the lipoxygenase inhibitor nordihydroguaiaretic acid. Analysis by gas chromatography combined with chemical ionization and electron impact mass spectrometry of reduced and nonreduced derivatives of the metabolite, indicate that the compound is 12-hydroxyeicosatetraenoic acid. Fractions of isolated microvessels enriched with capillaries produce 2.1 times more 12-hydroxyeicosatetraenoic acid per microgram of protein than do fractions of microvessels enriched with arterioles. These studies confirm that brain microvessels can produce 12-hydroxyeicosatetraenoic acid and strongly suggest that cerebral endothelia are the primary source of microvessel-derived 12-hydroxyeicosatetraenoic acid. They further suggest that in brain injury, the liberation and accumulation of arachidonic acid in cerebral tissues may lead to the production of 12-hydroxyeicosatetrae...Continue Reading

References

May 1, 1987·Journal of Neurochemistry·T ShimizuY Seyama
Nov 1, 1987·Neurochemical Research·G S HambrechtE F Ellis
Jan 1, 1988·The American Journal of Physiology·S A MooreM N Hart
Aug 1, 1987·The American Journal of Physiology·J A Gordon, A A Spector
Jun 1, 1986·Prostaglandins·C Hadjiagapiou, A A Spector
Feb 28, 1985·Biochemical and Biophysical Research Communications·A BabaH Iwata
Jul 31, 1985·Biochemical and Biophysical Research Communications·Y HashimotoH Oka
Sep 1, 1985·Journal of Neurochemistry·S A AdesuyiE F Ellis
Sep 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Hamberg, B Samuelsson
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J A LindgrenB Samuelsson
Feb 17, 1984·Biochimica Et Biophysica Acta·M L Caspers, C A Diglio
Jan 1, 1983·Annals of Neurology·M E Raichle
Aug 1, 1984·Prostaglandins·T YoshimotoF Dray

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Citations

Jan 1, 1991·Neuroradiology·J Greenwood
Jan 1, 1991·Neurochemistry International·N C SchaadM Schorderet
Aug 1, 1995·Progress in Neurobiology·H Katsuki, S Okuda
Mar 1, 1995·Cellular Signalling·R E CatalánF Hernández
Mar 31, 2005·Microvascular Research·Ping ChenXiang Fang
Jan 1, 1994·Journal of Ocular Pharmacology·T AsakuraH Shichi
Mar 1, 1993·European Journal of Biochemistry·T WatanabeB Samuelsson
Dec 4, 2012·American Journal of Physiology. Heart and Circulatory Physiology·Lawan SiangjongWilliam B Campbell
May 29, 2004·Prostaglandins & Other Lipid Mediators·Ping ChenXiang Fang
Oct 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·S Hyslop, G De Nucci
May 3, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Junping YouRobert M Bryan
Jun 20, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Xiang FangArthur A Spector
Jun 19, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·F M FaraciN L Weintraub
Feb 13, 2001·American Journal of Physiology. Heart and Circulatory Physiology·M H ZinkN L Weintraub
Feb 10, 1991·Biochemical and Biophysical Research Communications·H Y KimN Salem

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