Brain natriuretic peptide is present in the human amniotic fluid and is secreted from amnion cells

The Journal of Clinical Endocrinology and Metabolism
H ItohM Mukoyama

Abstract

The presence and biochemical characteristics of human brain natriuretic peptide (hBNP) in the amniotic fluid at various gestational ages were investigated. The hBNP-like immunoreactivity (hBNP-LI) levels in amniotic fluid, determined by RIA, were 118.7 +/- 57.6 pmol/L (mean +/- SEM; n = 5) and 107.7 +/- 8.7 pmol/L (n = 9) in the first and second trimesters of pregnancy, respectively; it was significantly decreased to 28.4 +/- 5.1 pmol/L (n = 9) in the third trimester. However, human atrial natriuretic peptide-like immunoreactivity (hANP-LI) was not detected (< 0.3 pmol/L) in any of these samples. Northern blot analysis demonstrated hBNP mRNA in human amnion tissue. Moreover, cultured amnion cells secreted a significant amount of hBNP-LI (100-200 fmol/10(6) cells/day), but not hANP-LI, into the culture medium. The synthesis of hBNP in cultured amnion cells was further confirmed using the polymerase chain reaction. High performance gel permeation chromatography of hBNP-LI extracted from human amniotic fluid and the culture medium of amnion cells revealed that the predominant molecular form of hBNP-LI in both samples was the hBNP precursor, with an approximate mol wt of 12 kilodaltons. These findings indicate that hBNP is present ...Continue Reading

Citations

Mar 10, 2007·American Journal of Obstetrics and Gynecology·Walter C Lubbers, Pirooz Eghtesady
Sep 19, 2002·American Journal of Obstetrics and Gynecology·Keith W MarvinMurray D Mitchell
Dec 20, 2000·American Journal of Obstetrics and Gynecology·I A BuhimschiC P Weiner
Sep 11, 2016·Clinical Biochemistry·Jorge Andrés CarvajalAna María Delpiano
Jul 13, 2018·Circulation Journal : Official Journal of the Japanese Circulation Society·Takekazu MiyoshiNaoto Minamino
Oct 17, 2009·Molecular Reproduction and Development·Chenjiang YaoZhongdong Qiao
Apr 6, 2021·Frontiers in Physiology·Takekazu MiyoshiNaoto Minamino
Aug 10, 2021·The EPMA Journal·Adriana Torres CrignaOlga Golubnitschaja

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