Brain pericytes from stress-susceptible pigs increase blood-brain barrier permeability in vitro.

Fluids and Barriers of the CNS
Elodie VandenhauteMarie-Magdeleine Ruchoux

Abstract

The function of pericytes remains questionable but with improved cultured technique and the use of genetically modified animals, it has become increasingly clear that pericytes are an integral part of blood-brain barrier (BBB) function, and the involvement of pericyte dysfunction in certain cerebrovascular diseases is now emerging. The porcine stress syndrome (PSS) is the only confirmed, homologous model of malignant hyperthermia (MH) in veterinary medicine. Affected animals can experience upon slaughter a range of symptoms, including skeletal muscle rigidity, metabolic acidosis, tachycardia and fever, similar to the human syndrome. Symptoms are due to an enhanced calcium release from intracellular stores. These conditions are associated with a point mutation in ryr1/hal gene, encoding the ryanodine receptor, a calcium channel. Important blood vessel wall muscle modifications have been described in PSS, but potential brain vessel changes have never been documented in this syndrome. In the present work, histological and ultrastructural analyses of brain capillaries from wild type and ryr1 mutated pigs were conducted to investigate the potential impairment of pericytes, in this pathology. In addition, brain pericytes were isolate...Continue Reading

References

May 1, 1990·Journal of Neurochemistry·M P DehouckR Cecchelli
Jul 1, 1987·Journal of Cellular Physiology·A Siflinger-BirnboimA B Malik
Mar 21, 1998·The Journal of Cell Biology·S EsserW Risau
Jun 6, 2000·Advanced Drug Delivery Reviews·R CecchelliM P Dehouck
Oct 23, 2001·Developmental Dynamics : an Official Publication of the American Association of Anatomists·U OzerdemW B Stallcup
Sep 17, 2005·Circulation Research·Annika ArmulikChrister Betsholtz
Oct 13, 2006·Nature·Claire M PeppiattDavid Attwell
Aug 2, 2007·Nature Reviews. Drug Discovery·Romeo CecchelliLaurence Fenart
Sep 8, 2007·Cellular and Molecular Neurobiology·Shinsuke NakagawaMasami Niwa
Dec 30, 2008·Neurochemistry International·Shinsuke NakagawaMasami Niwa
Sep 21, 2010·Journal of the Neurological Sciences·J M Wardlaw
Oct 15, 2010·Nature·Annika ArmulikChrister Betsholtz
Nov 18, 2010·Methods in Molecular Biology·Sukriti Nag
Feb 26, 2011·Fluids and Barriers of the CNS·Drew BonkowskiPaula Dore-Duffy
May 24, 2011·The International Journal of Biochemistry & Cell Biology·Gokulan ThanabalasundaramHans-Joachim Galla
Sep 14, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Malou P H SchreursMarilyn J Cipolla
Oct 26, 2011·Current Neurovascular Research·Elodie VandenhauteMarie-Pierre Dehouck
Oct 28, 2011·Nature Neuroscience·Ethan A WinklerBerislav V Zlokovic
Dec 7, 2011·Methods in Molecular Biology·Vladimir Katyshev, Paula Dore-Duffy
Apr 10, 2012·Microvascular Research·Ulrich TiggesRichard Milner

❮ Previous
Next ❯

Citations

Feb 26, 2013·In Vitro Cellular & Developmental Biology. Animal·Siti-Nazrina CamalxamanMohd Lila Mohd-Azmi
Mar 20, 2014·Molecular Pharmaceutics·Imola Wilhelm, István A Krizbai
Feb 3, 2016·Frontiers in Molecular Neuroscience·Petra SánthaMária A Deli
Jul 17, 2014·Journal of Medicinal Chemistry·N V S Dinesh K BhupathirajuM Graça H Vicente
Jan 10, 2018·Frontiers in Molecular Neuroscience·Hyung Joon ChoMichal Toborek
Feb 16, 2018·Clinical Science·Isobel ParkesM Zameel Cader
Apr 16, 2013·American Journal of Physiology. Heart and Circulatory Physiology·Paulo W PiresAnne M Dorrance
Mar 13, 2021·PloS One·Maj Schneider ThomsenLouiza Bohn Thomsen

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electron microscopy

Software Mentioned

GraphPad
GraphPad Prism
Adobe Photoshop

Related Concepts

Related Feeds

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.