PMID: 6989447Apr 21, 1980Paper

Brain primary culture - a characterization

Brain Research
E HanssonL Rönnbäck

Abstract

Primary cultures from rat or mouse brain hemispheres contain predominantly glial cells. These cells accumulated [3H]GABA and showed a specific fluorescence with FITC-labeled anti-S-100 antiserum. Round or elongated cells which reacted positively with antimacrophage antiserum and exhibited phagocytotic activity, were considered mesodermal macrophages. Big flat cells, unreactive to S-100 antiserum or to any other antiserum tested, showed a morphology similar to mesenchymal cells of a low differentiation grade. These cells formed a monolayer upon which other cell types grew. Positive reaction for alkaline phosphatases was used as a criterion to identify endothelial cells, the number of which increased with increasing age of the cultures. Anti-14-3-2 antiserum gave no specific reaction, indicating the presence of very few or no differentiated neurons in the cultures.

References

Apr 1, 1977·Brain Research·A GrassoV J Perez
Feb 1, 1978·Journal of the Neurological Sciences·L I PerssonH Nygren
Dec 26, 1975·Brain Research·H Hydén, L Rönnbäck
Jul 1, 1973·Journal of Neurochemistry·L DittmannP Mandel
Sep 1, 1956·The American Journal of Anatomy·I COSTERO, C M POMERAT
Jan 1, 1963·Zeitschrift für Zellforschung und mikroskopische Anatomie·S S VARONC M POMERAT
Feb 19, 1964·Zeitschrift für Zellforschung und mikroskopische Anatomie·H A HANSSON, P SOURANDER

Citations

May 1, 1984·Neurochemical Research·E HanssonA Sellström
Mar 1, 1983·Neurochemical Research·E Hansson, L Rönnbäck
Feb 1, 1992·Molecular and Chemical Neuropathology·P Rodríguez-PomboM Ugarte
Feb 1, 1984·Journal of Neuroscience Methods·J W KoperL M van Golde
Jan 1, 1982·Journal of Neuroimmunology·P G Kennedy
Dec 30, 1983·Neuroscience Letters·S J LolaitB H Toh
Jan 1, 1984·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I Holopainen
Aug 1, 1984·Acta Physiologica Scandinavica·E HanssonA Sellström
Jul 1, 1984·Journal of Neurochemistry·G E PfyfferC W Heizmann
Jan 1, 1984·Journal of Neurochemistry·J E Goldman, F C Chiu
Feb 1, 1984·Neurochemical Research·E Hansson
Jan 1, 1983·Journal of Neurochemistry·E Hansson, A Sellström
Feb 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A C YuP H Chan
Jan 1, 1983·Journal of Neuroscience Research·E Hansson, L Rönnbäck
Oct 1, 1981·Scandinavian Journal of Immunology·L I PerssonL Rönnbäck
Sep 13, 2008·Neurochemical Research·Radovan MurínBernd Hamprecht

Related Concepts

Alkaline Phosphatase
Brain
Cell Division Phases
Cell Survival
Immunofluorescence Assay
Gamma-Aminobutyric Acid, Zinc Salt (2:1)
Phagocytosis

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