PMID: 6989447Apr 21, 1980Paper

Brain primary culture - a characterization

Brain Research
E HanssonL Rönnbäck

Abstract

Primary cultures from rat or mouse brain hemispheres contain predominantly glial cells. These cells accumulated [3H]GABA and showed a specific fluorescence with FITC-labeled anti-S-100 antiserum. Round or elongated cells which reacted positively with antimacrophage antiserum and exhibited phagocytotic activity, were considered mesodermal macrophages. Big flat cells, unreactive to S-100 antiserum or to any other antiserum tested, showed a morphology similar to mesenchymal cells of a low differentiation grade. These cells formed a monolayer upon which other cell types grew. Positive reaction for alkaline phosphatases was used as a criterion to identify endothelial cells, the number of which increased with increasing age of the cultures. Anti-14-3-2 antiserum gave no specific reaction, indicating the presence of very few or no differentiated neurons in the cultures.

References

Jun 30, 1975·Annals of the New York Academy of Sciences·P Brandtzaeg
Apr 1, 1977·Brain Research·A GrassoV J Perez
Feb 1, 1978·Journal of the Neurological Sciences·L PerssonH Nygren
Dec 26, 1975·Brain Research·H Hydén, L Rönnbäck
Jul 1, 1973·Journal of Neurochemistry·L DittmannP Mandel
Sep 1, 1956·The American Journal of Anatomy·I COSTERO, C M POMERAT
Jan 1, 1963·Zeitschrift für Zellforschung und mikroskopische Anatomie·S S VARONC M POMERAT
Feb 19, 1964·Zeitschrift für Zellforschung und mikroskopische Anatomie·H A HANSSON, P SOURANDER

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Citations

May 1, 1984·Neurochemical Research·E HanssonA Sellström
Mar 1, 1983·Neurochemical Research·E Hansson, L Rönnbäck
Feb 1, 1992·Molecular and Chemical Neuropathology·P Rodríguez-PomboM Ugarte
Feb 1, 1984·Journal of Neuroscience Methods·J W KoperL M van Golde
Jan 1, 1982·Journal of Neuroimmunology·P G Kennedy
Dec 30, 1983·Neuroscience Letters·S J LolaitB H Toh
Jan 1, 1984·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·I Holopainen
Aug 1, 1984·Acta Physiologica Scandinavica·E HanssonA Sellström
Jul 1, 1984·Journal of Neurochemistry·G E PfyfferC W Heizmann
Jan 1, 1984·Journal of Neurochemistry·J E Goldman, F C Chiu
Feb 1, 1984·Neurochemical Research·E Hansson
Jan 1, 1983·Journal of Neurochemistry·E Hansson, A Sellström
Feb 1, 1989·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·A C YuP H Chan
Jan 1, 1983·Journal of Neuroscience Research·E Hansson, L Rönnbäck
Oct 1, 1981·Scandinavian Journal of Immunology·L I PerssonL Rönnbäck
Sep 13, 2008·Neurochemical Research·Radovan MurínBernd Hamprecht
Jan 7, 1982·Brain Research·E HanssonA Sellström

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