Brain-specific regulator of G-protein signaling 9-2 selectively interacts with alpha-actinin-2 to regulate calcium-dependent inactivation of NMDA receptors

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Mohamad BouhamdanRodrigo Andrade

Abstract

Regulator of G-protein signaling 9-1 (RGS9-1) and RGS9-2 are highly related RGS proteins with distinctive C termini arising from alternative splicing of RGS9 gene transcripts. RGS9-1 is expressed in photoreceptors where it functions as a regulator of transducin. In contrast, RGS9-2 is abundantly expressed in the brain, especially in basal ganglia, where its specific function remains poorly understood. To gain insight into the function of RGS9-2, we screened a human cDNA library for potential interacting proteins. This screen identified a strong interaction between RGS9-2 and alpha-actinin-2, suggesting a possible functional relationship between these proteins. Consistent with this idea, RGS9-2 and alpha-actinin-2 coimmunoprecipitated after coexpression in human embryonic kidney 293 (HEK-293) cells. Furthermore, endogenous RGS9-2 and alpha-actinin-2 could also be coimmunoprecipitated from extracts of rat striatum, an area highly enriched in both these proteins. These results supported the idea that RGS9-2 and alpha-actinin-2 could act in concert in central neurons. Like alpha-actinin-2, RGS9-2 coimmunoprecipitated NMDA receptors from striatal extracts, suggesting an interaction between RGS9-2, alpha-actinin-2, and NMDA receptors...Continue Reading

Citations

Jun 13, 2009·Cell Biochemistry and Biophysics·Garret R AndersonKirill A Martemyanov
Jan 1, 2010·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Garret R AndersonKirill A Martemyanov
Aug 14, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Hai-Dun YanRodrigo Andrade
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