Brain-stiffness-mimicking tilapia collagen gel promotes the induction of dorsal cortical neurons from human pluripotent stem cells

Scientific Reports
Misato IwashitaYoichi Kosodo

Abstract

The mechanical properties of the extracellular microenvironment, including its stiffness, play a crucial role in stem cell fate determination. Although previous studies have demonstrated that the developing brain exhibits spatiotemporal diversity in stiffness, it remains unclear how stiffness regulates stem cell fate towards specific neural lineages. Here, we established a culture substrate that reproduces the stiffness of brain tissue using tilapia collagen for in vitro reconstitution assays. By adding crosslinkers, we obtained gels that are similar in stiffness to living brain tissue (150-1500 Pa). We further examined the capability of the gels serving as a substrate for stem cell culture and the effect of stiffness on neural lineage differentiation using human iPS cells. Surprisingly, exposure to gels with a stiffness of approximately 1500 Pa during the early period of neural induction promoted the production of dorsal cortical neurons. These findings suggest that brain-stiffness-mimicking gel has the potential to determine the terminal neural subtype. Taken together, the crosslinked tilapia collagen gel is expected to be useful in various reconstitution assays that can be used to explore the role of stiffness in neurogenesi...Continue Reading

References

Apr 1, 1996·Biomaterials·L H Olde DaminkJ Feijen
Apr 23, 2004·Trends in Neurosciences·Robert T FremeauRobert H Edwards
Dec 2, 2004·Cell Motility and the Cytoskeleton·Tony YeungPaul A Janmey
Oct 20, 2005·Journal of Bioscience and Bioengineering·Shunji YunokiMasanobu Munekata
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Nov 10, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yun-Bi LuAndreas Reichenbach
May 24, 2007·Journal of Neurotrauma·Benjamin S ElkinBarclay Morrison
Jul 29, 2008·Biophysical Journal·Krishanu SahaKevin E Healy
Jan 24, 2009·Nature Reviews. Cancer·Darci T ButcherValerie M Weaver
Jan 27, 2009·Cell Motility and the Cytoskeleton·Ektoras HadjipanayiRobert A Brown
Jan 31, 2009·Proceedings of the National Academy of Sciences of the United States of America·Seunghan OhSungho Jin
Aug 21, 2009·Cell and Tissue Research·Malgorzata BarczykDonald Gullberg
Sep 2, 2009·Biophysical Journal·Brett C IsenbergJoyce Y Wong
Jun 4, 2010·Current Protocols in Cell Biology·Justin R Tse, Adam J Engler
Jun 9, 2010·Journal of Biomechanical Engineering·Benjamin S ElkinBarclay Morrison
Nov 16, 2010·Nature Methods·Joseph R KlimLaura L Kiessling
Dec 24, 2010·Journal of Visualized Experiments : JoVE·Yvonne Aratyn-SchausMargaret L Gardel
Feb 18, 2011·Disease Models & Mechanisms·Thomas R Cox, Janine T Erler
Mar 10, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Pouria MoshayediKristian Franze
Aug 3, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Albert J KeungDavid V Schaffer
Aug 6, 2013·Cell Stem Cell·Naohisa YoshiokaSteven F Dowdy
May 9, 2014·BioMed Research International·Kohei YamamotoYoshihiko Hayashi
Jul 2, 2014·BioMed Research International·Shizuka YamadaYoshihiko Hayashi
Sep 2, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Ganesan KrishnamoorthyMukesh Doble
Sep 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Samira MusahLaura L Kiessling
Nov 11, 2014·Nature Neuroscience·Marco OnoratiElena Cattaneo
Aug 6, 2015·Journal of Food Science and Technology·Fazli SubhanAbdul Ghafoor

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Citations

Apr 1, 2020·Frontiers in Cell and Developmental Biology·Naotaka Nakazawa, Mineko Kengaku
Aug 4, 2020·Journal of Alzheimer's Disease : JAD·Jieun KimJaewon Lee
Nov 6, 2020·Biomaterials Science·Kamol DeyLuciana Sartore
Oct 20, 2020·Medical Hypotheses·Silvano Traverso
Apr 23, 2021·Neurochemistry International·Janko KajtezJenny Emnéus
May 5, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Hayato L MizunoYuki Akagi
Nov 10, 2021·Biomacromolecules·Kelti A SmithMolly S Shoichet
Jan 14, 2022·Biomaterials Science·Alp OzgunFabio Variola

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Methods Mentioned

BETA
chemical modification
scanning electron microscopy
circular dichroism

Software Mentioned

Graphpad
Prism

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