Brain to blood efflux transport of adenosine: blood-brain barrier studies in the rat

Journal of Neurochemistry
Aleksandra J IsakovicZoran B Redzic

Abstract

The blood-brain barrier (BBB) efflux transport of [(14)C] adenosine was studied using the brain efflux index (BEI) technique. BEI increased linearly over the first 2 min after injection, with deviation from linearity thereafter; 90.12 +/- 1.5% of the injected [(14)C] radioactivity remained within the brain after 20 min. The remaining tracer appears to be mainly intracellular, trapped by phosphorylation, as an almost linear increase of BEI over 20 min was observed after intracerebral injection of [(14)C] adenosine together with 5-iodo tubercidin. The BBB efflux clearance of [(14)C] radioactivity was estimated to be 27.62 +/- 5.2 micro L/min/g, almost threefold higher than the BBB influx clearance estimated by the brain uptake index technique. High-performance liquid chromatography (HPLC) analysis of blood plasma collected from the jugular vein after the intracerebral injection revealed metabolic breakdown of [(14)C] adenosine into nucleobases. The BBB efflux transport was saturable with apparent K(m) = 13.22 +/- 1.75 micro m and V(max) = 621.07 +/- 71.22 pmole/min/g, which indicated that BBB efflux in vivo is 6.2-12p mole/min/g, negligible when compared to the reported rate of adenosine uptake into neurones/glia. However, these ...Continue Reading

Citations

Jul 2, 2009·Pharmaceutical Research·Ken-ichi Hosoya, Masanori Tachikawa
Feb 13, 2010·Cerebrospinal Fluid Research·Zoran B RedzicAleksandra J Isakovic
Aug 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Laura B JaegerAndrew V Schally
Dec 30, 2011·Brain Structure & Function·Sylvia Van DorpeBart De Spiegeleer
Aug 30, 2008·Neurochemical Research·Zoran B RedzicAleksandra J Isakovic
Aug 16, 2008·Respiratory Physiology & Neurobiology·Gaspard MontandonAida Bairam
Feb 27, 2014·Scientific Reports·Junko MutoToshio Mikami
Feb 21, 2006·Biochimica Et Biophysica Acta·Katsuhiko NagaseKen-Ichi Hosoya
May 22, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·Cláudia SaraivaLiliana Bernardino
Nov 2, 2016·The Journal of Physiology·Stanislaw TubekPiotr Ponikowski
Mar 13, 2016·The Neuroscientist : a Review Journal Bringing Neurobiology, Neurology and Psychiatry·Weihong Pan, Abba J Kastin
Nov 25, 2014·Nature Nanotechnology·Alice GaudinPatrick Couvreur
Feb 6, 2019·Journal of Drug Targeting·Simona MuraJulien Nicolas
Dec 18, 2013·Pharmacological Reviews·Geoffrey Burnstock, Vera Ralevic
Jun 17, 2020·Neuroscience Bulletin·Bruno G Frenguelli, Nicholas Dale

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