Brain transcriptional stability upon prion protein-encoding gene invalidation in zygotic or adult mouse.

BMC Genomics
Sead ChadiJean-Luc Vilotte

Abstract

The physiological function of the prion protein remains largely elusive while its key role in prion infection has been expansively documented. To potentially assess this conundrum, we performed a comparative transcriptomic analysis of the brain of wild-type mice with that of transgenic mice invalidated at this locus either at the zygotic or at the adult stages. Only subtle transcriptomic differences resulting from the Prnp knockout could be evidenced, beside Prnp itself, in the analyzed adult brains following microarray analysis of 24 109 mouse genes and QPCR assessment of some of the putatively marginally modulated loci. When performed at the adult stage, neuronal Prnp disruption appeared to sequentially induce a response to an oxidative stress and a remodeling of the nervous system. However, these events involved only a limited number of genes, expression levels of which were only slightly modified and not always confirmed by RT-qPCR. If not, the qPCR obtained data suggested even less pronounced differences. These results suggest that the physiological function of PrP is redundant at the adult stage or important for only a small subset of the brain cell population under classical breeding conditions. Following its early repor...Continue Reading

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Citations

Aug 21, 2013·Molecular Neurobiology·Franc LlorensJosé Antonio del Río
Mar 2, 2016·The Journal of Experimental Medicine·Mario NuvoloneAdriano Aguzzi
May 4, 2013·Journal of Neurochemistry·Franc LlorensJosé A del Río
Nov 5, 2014·Frontiers in Cell and Developmental Biology·Sophie HalliezVincent Béringue
Nov 5, 2014·Frontiers in Cell and Developmental Biology·Samira MakzhamiJean-Luc Vilotte
Jun 27, 2017·PloS One·Giulia MalachinMaren K Bakkebø
Nov 1, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Damien LoubetBenoit Schneider
Mar 17, 2011·Physiological Genomics·Stefano BenvegnùGiuseppe Legname
May 4, 2017·BMC Biology·Marie-Angela WulfAdriano Aguzzi
Apr 30, 2021·Frontiers in Cellular Neuroscience·Benoit SchneiderJean-Marie Launay

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Datasets Mentioned

BETA
AV451297.1

Methods Mentioned

BETA
PCR
transgenic
gene knockdown
biopsies

Software Mentioned

Ingenuity
GENEPIX

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