Branched-chain amino acid and lysine deficiencies exert different effects on mammary translational regulation

Journal of Dairy Science
J DoelmanJ P Cant

Abstract

Deficiencies and imbalances of specific group II essential amino acids (EAA) were created in lactating cows by an infusion subtraction protocol to explore effects on milk production and abundance and phosphorylation state of regulators of mRNA translation in the mammary glands. Five lactating cows on a diet of 11.2% crude protein were infused abomasally for 5d with saline, 563 g/d of a complete EAA mix, or EAA mixes without the branched-chain amino acids (BCAA), Leu, or Lys in a 5 × 5 Latin square design. Milk protein yield was stimulated by EAA infusion and returned to saline levels upon subtraction of BCAA, Leu, or Lys. Mammary abundance of phosphorylated S6K1 was measured as an indicator of mammalian target of rapamycin complex 1 (mTORC1) activity and was found not to be affected by the complete EAA mix but was increased by the mixture lacking Lys. Total S6K1 abundances in mammary tissue were elevated by complete and BCAA-lacking infusions. All of the EAA treatments except the one lacking BCAA upregulated mammary eIF2Bε and eIF2α abundances, which is stimulatory to global mRNA translation. Phosphorylation state of eIF2Bε tended to decrease when complete or Lys-lacking EAA mixtures were infused. Phosphorylation state of eIF2α...Continue Reading

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Citations

Jan 28, 2017·Journal of Animal Science and Biotechnology·Shihai ZhangShiyan Qiao
Apr 2, 2018·Journal of Dairy Science·John P CantJohn Doelman
Oct 1, 2019·Endocrinology and Metabolism·Md Abu Bakkar Siddik, Andrew C Shin
Dec 10, 2020·Journal of Animal Science and Biotechnology·Virginia L Pszczolkowski, Sebastian I Arriola Apelo
Nov 24, 2020·Journal of Dairy Science·Virginia L PszczolkowskiSebastian I Arriola Apelo
May 1, 2021·Cancers·Melina de Moraes Santos OliveiraMaria Cristina Cintra Gomes-Marcondes
Aug 8, 2021·Animals : an Open Access Journal From MDPI·Jung-Eun Kim, Hong-Gu Lee

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