Branched chain amino acids activate messenger ribonucleic acid translation regulatory proteins in human skeletal muscle, and glucocorticoids blunt this action

The Journal of Clinical Endocrinology and Metabolism
Z LiuE J Barrett

Abstract

Branched chain amino acids (BCAA) are particularly effective anabolic agents. Recent in vitro studies suggest that amino acids, particularly leucine, activate a signaling pathway that enhances messenger ribonucleic acid translation and protein synthesis. The physiological relevance of these findings to normal human physiology is uncertain. We examined the effects of BCAA on the phosphorylation of eukaryotic initiation factor 4E-binding protein 1 (eIF4E-BP1) and ribosomal protein S6 kinase (p70(S6K)) in skeletal muscle of seven healthy volunteers. We simultaneously examined whether BCAA affect urinary nitrogen excretion and forearm skeletal muscle protein turnover and whether the catabolic action of glucocorticoids could be mediated in part by inhibition of the action of BCAA on the protein synthetic apparatus. BCAA infusion decreased urinary nitrogen excretion (P < 0.02), whole body phenylalanine flux (P < 0.02), plasma phenylalanine concentration (P < 0.001), and improved forearm phenylalanine balance (P = 0.03). BCAA also increased the phosphorylation of both eIF4E-BP1 (P < 0.02) and p70(S6K) (P < 0.03), consistent with an action to activate the protein synthetic apparatus. Dexamethasone increased plasma phenylalanine concent...Continue Reading

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Citations

Sep 10, 2009·American Journal of Physiology. Endocrinology and Metabolism·Kevin R ShortK Sreekumaran Nair
Dec 7, 2007·American Journal of Physiology. Endocrinology and Metabolism·Hans C DreyerBlake B Rasmussen
Jan 10, 2008·The Journal of Clinical Endocrinology and Metabolism·Guolian LiZhenqi Liu
Nov 26, 2008·BMC Biochemistry·Simon S Wing
Aug 11, 2004·International Journal of Obesity and Related Metabolic Disorders : Journal of the International Association for the Study of Obesity·P DeibertA Berg

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