Branched-chain and aromatic amino acid catabolism into aroma volatiles in Cucumis melo L. fruit

Journal of Experimental Botany
Itay GondaEfraim Lewinsohn


The unique aroma of melons (Cucumis melo L., Cucurbitaceae) is composed of many volatile compounds biosynthetically derived from fatty acids, carotenoids, amino acids, and terpenes. Although amino acids are known precursors of aroma compounds in the plant kingdom, the initial steps in the catabolism of amino acids into aroma volatiles have received little attention. Incubation of melon fruit cubes with amino acids and alpha-keto acids led to the enhanced formation of aroma compounds bearing the side chain of the exogenous amino or keto acid supplied. Moreover, L-[(13)C(6)]phenylalanine was also incorporated into aromatic volatile compounds. Amino acid transaminase activities extracted from the flesh of mature melon fruits converted L-isoleucine, L-leucine, L-valine, L-methionine, or L-phenylalanine into their respective alpha-keto acids, utilizing alpha-ketoglutarate as the amine acceptor. Two novel genes were isolated and characterized (CmArAT1 and CmBCAT1) encoding 45.6 kDa and 42.7 kDa proteins, respectively, that displayed aromatic and branched-chain amino acid transaminase activities, respectively, when expressed in Escherichia coli. The expression of CmBCAT1 and CmArAT1 was low in vegetative tissues, but increased in fles...Continue Reading


Nov 1, 1990·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E Poulsen
May 1, 1990·The Journal of Speech and Hearing Disorders·L E Humes, E U Kirn
Jan 2, 1995·Biochimica Et Biophysica Acta·G E SéraliniF Labrie
Dec 14, 1999·Protein Science : a Publication of the Protein Society·W BlankenfeldtH J Hecht
Feb 7, 2001·The Journal of Biological Chemistry·J Richard DickinsonMichael J E Hewlins
Aug 24, 2000·Journal of Agricultural and Food Chemistry·S G Wyllie, J K Fellman
Mar 23, 2001·Journal of Agricultural and Food Chemistry·M ShalitEfraim Lewinsohn
Mar 27, 2001·Acta Crystallographica. Section D, Biological Crystallography·N YennawarG Farber
Apr 21, 2001·Journal of Agricultural and Food Chemistry·J C Beaulieu, C C Grimm
Aug 30, 2001·Acta Crystallographica. Section A, Foundations of Crystallography·G Fadda, G Zanzotto
Dec 18, 2001·Journal of Agricultural and Food Chemistry·M J JordánK L Goodner
Jun 27, 2002·Journal of Agricultural and Food Chemistry·Ana G PérezCarlos Sanz
Dec 25, 2002·The Journal of Biological Chemistry·J Richard DickinsonMichael J E Hewlins
Mar 6, 2003·Neuroscience Letters·Christian RamakersAntoon F M Moorman
Aug 7, 2003·Applied and Environmental Microbiology·Zeynep VuralhanJack T Pronk
Sep 2, 2003·EMBO Reports·Riccardo Percudani, Alessio Peracchi
Aug 3, 2004·Plant Physiology·Jennifer BoatrightNatalia Dudareva
Aug 25, 2004·Plant Physiology·Jules BeekwilderAsaph Aharoni
Mar 28, 2006·Phytochemistry·Mwafaq IbdahEfraim Lewinsohn
May 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Denise M TiemanHarry J Klee
Aug 26, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·M MalatrasiN Marmiroli
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Wilfried SchwabEfraim Lewinsohn
Jun 10, 2008·Applied and Environmental Microbiology·Mengjin LiuRoland J Siezen
Jul 23, 2008·The Plant Journal : for Cell and Molecular Biology·Rachel Davidovich-RikanatiYaron Sitrit
Dec 20, 2008·Hospitals & Health Networks·Beth Friedman
Jun 1, 1991·Plant Cell Reports·B M Reed


Mar 26, 2011·Journal of Experimental Botany·Andrej Kochevenko, Alisdair R Fernie
May 5, 2012·Annual Review of Plant Biology·Hiroshi Maeda, Natalia Dudareva
Jan 22, 2014·Journal of Experimental Botany·Takayuki TohgeAlisdair R Fernie
Dec 5, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Gaihua QinShaoling Zhang
Feb 3, 2016·Amino Acids·Korey KilpatrickFrédéric Marsolais
Feb 3, 2016·Scientific Reports·Hiroshi HirataNaoharu Watanabe
Apr 13, 2013·Food Chemistry·Francisco J HidalgoRosario Zamora
Dec 19, 2012·Trends in Biotechnology·David ToubianaAaron Fait
Apr 17, 2012·Trends in Plant Science·Jillian M HagelPeter J Facchini
Jan 10, 2012·Journal of Plant Physiology·Andrej KochevenkoWagner L Araújo
Oct 17, 2015·Food Chemistry·Xiaotang YangZhaoqi Zhang
Sep 11, 2013·Biotechnology Journal·Irene Aragüez, Victoriano Valpuesta
Feb 14, 2013·The Plant Journal : for Cell and Molecular Biology·Itay GondaEfraim Lewinsohn
Feb 7, 2013·The New Phytologist·Natalia DudarevaIan Kaplan
Jul 28, 2015·Journal of the Science of Food and Agriculture·Libia A Chaparro-TorresJuan Pablo Fernández-Trujillo
Jul 21, 2015·Journal of Agricultural and Food Chemistry·Yanyan ZhangHolger Zorn
Oct 3, 2014·Bioscience, Biotechnology, and Biochemistry·Ruijie HaoQixiang Zhang
Jan 7, 2016·Annual Review of Plant Biology·Gad GaliliAlisdair R Fernie
Jun 15, 2016·Plant Science : an International Journal of Experimental Plant Biology·Willian Batista SilvaWagner L Araújo
Jun 15, 2016·Bioscience, Biotechnology, and Biochemistry·Hiroshi HirataNaoharu Watanabe
Aug 1, 2016·Trends in Plant Science·Pulu SunSylvie Baudino
Nov 4, 2016·Plant Signaling & Behavior·Sam D Cook, John J Ross
Feb 1, 2018·The Plant Journal : for Cell and Molecular Biology·Navot GalpazNurit Katzir
Oct 6, 2017·The New Phytologist·Katherine R Goodrich, Andreas Jürgens
May 3, 2018·Annals of Botany·Bertrand BeauvoitYves Gibon
Dec 18, 2018·The Plant Journal : for Cell and Molecular Biology·Minmin WangFlorence Zakharov
Jun 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min Kyung Park, Young-Suk Kim
Mar 24, 2018·Journal of Food and Drug Analysis·Robert J Cannon, Chi-Tang Ho
Apr 17, 2020·Journal of the Science of Food and Agriculture·Hnin Phyu Lwin, Jinwook Lee
Jul 28, 2020·Journal of the Science of Food and Agriculture·Mohamed ZaridJuan Pablo Fernández-Trujillo
Apr 24, 2018·Frontiers in Molecular Biosciences·Anutthaman ParthasarathyAndré O Hudson
Jul 12, 2019·Frontiers in Plant Science·Romina PedreschiReinaldo Campos-Vargas
Dec 2, 2020·International Journal of Molecular Sciences·Jesús Picazo-AragonésFrancisco Balao
Jan 13, 2021·Proceedings of the National Academy of Sciences of the United States of America·Nobuko SugimotoRandolph Beaudry

Related Concepts

Amino Acids, Branched-Chain
Plant Proteins
Physical Process of Vaporization
Gene Expression Regulation, Plant
Amino Acids, Aromatic
Escherichia coli
Fatty Acids

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.