Aug 11, 2014

Hepatic NFAT signaling regulates the expression of inflammatory cytokines in cholestasis

BioRxiv : the Preprint Server for Biology
Jomar Fajardo RabajanteJames L Boyer

Abstract

The inflammatory response plays an important role in cholestatic liver injury where bile acid (BA) induction of proinflammatory cytokines in hepatocytes is an initial pathophysiologic event. However, the signaling pathways involving BA stimulation of cytokine production remain to be elucidated. In this report, we examined the functional role of the Nuclear Factor of Activated T-cells (NFAT) in BA-induction of inflammatory genes in hepatic cells and cholestatic livers. We found that NFAT isoform c1 and c3 were expressed in human and mouse hepatocytes. When treated with cholestatic levels of BA, both human and mouse hepatocytes but not cholangiocytes increased NFATc3 nuclear translocation, associated with elevated mRNA levels of IL-8, Cxcl2, and Cxcl10 in these cells. Blocking NFAT activation with pathway-specific inhibitors (i.e. cyclosporine A, FK-506, KN-62 and Inca-6) or knocking down Nfatc3, significantly repressed BA-induction of these cytokines in mouse hepatocytes, including Ccl2, Cxcl2, Cxcl10, Icam1 and Egr1. Nuclear expression of NFATc3/Nfatc3 protein was also increased in cholestatic livers after bile duct ligation or in Abcb4-/- mice and in patients with primary biliary cholangitis and primary sclerosing cholangitis ...Continue Reading

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Mentioned in this Paper

Potassium Channel Opener [EPC]
Cell Dedifferentiation
Transdifferentiation
2-Dimensional
Genes
Dedifferentiation
Determination Aspects
Cell Differentiation Process
Anaplasia
Study of Epigenetics

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