Brazilian Green Propolis Inhibits Ox-LDL-Stimulated Oxidative Stress in Human Umbilical Vein Endothelial Cells Partly through PI3K/Akt/mTOR-Mediated Nrf2/HO-1 Pathway

Evidence-based Complementary and Alternative Medicine : ECAM
Wenwen YuanHongzhuan Xuan

Abstract

Propolis has been widely used as a dietary supplement for its health benefits, including cardiovascular protective effects. The aim of this study was to investigate the cytoprotective effects of Brazilian green propolis (BP) against oxidized low-density lipoprotein (Ox-LDL) induced human umbilical vein endothelial cells (HUVECs) damage. Our results suggested that treatment with BP rescued Ox-LDL-stimulated HUVECs cell viability losses, which might be associated with its inhibitive effects on the cell apoptosis and autophagy. We also noticed that BP restored Ox-LDL-stimulated HUVECs oxidative stress, by induced antioxidant gene expressions, including Heme oxygenase-1 and its upstream mediator, Nrf2, which were mediated by the activation of the phosphorylation of PI3K/Akt/mTOR. Pretreatment with wortmannin, PI3K/AKT inhibitor, abolished BP induced Nrf2 nuclear translocation and HO-1 level. Our results demonstrated that BP protected HUVECs against oxidative damage partly via PI3K/Akt/mTOR-mediated Nrf/HO-1 pathway, which might be applied into preventing Ox-LDL mediated cardiovascular diseases.

References

Apr 28, 2004·Free Radical Biology & Medicine·Ken ItohMasayuki Yamamoto
Jul 5, 2005·Journal of Ethnopharmacology·Vassya Bankova
Sep 22, 2007·Phytotherapy Research : PTR·Tai-Hwan AhnJong-Choon Kim
Sep 18, 2008·Cell and Tissue Research·Anca V SimaMaya Simionescu
Jan 20, 2010·FEBS Letters·Chang Hwa JungDo-Hyung Kim
Jul 21, 2010·Biochemical Pharmacology·Ananta PaineStephan Immenschuh
Mar 3, 2011·Archives of Toxicology·Liam Baird, Albena T Dinkova-Kostova
Apr 23, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Haisha YangXiaobo Li
Jul 1, 2008·Environmental Toxicology and Pharmacology·Muneo MotomuraCheorl-Ho Kim
Aug 19, 2011·Molecular and Cellular Biochemistry·Chun-Yan WuLu-Shan Liu
Nov 9, 2011·Nature Medicine·Christian Weber, Heidi Noels
Nov 29, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Natalia S S GuimarãesTiago Rodrigues
Dec 30, 2011·Current Pharmaceutical Design·Tim Van-AsscheCharalambos Antoniades
Nov 20, 2013·International Journal of Biological Sciences·Peramaiyan RajendranIkuo Nishigaki
Dec 3, 2013·Natural Product Reports·Hemant KumarDong-Kug Choi
Nov 29, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shuai HuangFu-Liang Hu
Mar 25, 2015·Evidence-based Complementary and Alternative Medicine : ECAM·Jianglin ZhangFuliang Hu
Jan 11, 2017·BMC Research Notes·Ranjan PremaratnaT G A Nilmini Chandrasena
Oct 27, 2018·Journal of the American College of Cardiology·Jesse W WilliamsJason C Kovacic
Nov 8, 2018·Nutrients·Nour YahfoufiChantal Matar
Feb 7, 2019·Frontiers in Immunology·Yahya SohrabiHannes M Findeisen

❮ Previous
Next ❯

Citations

Feb 16, 2020·Environmental Toxicology·Zeynep M CoskunAynur Acar
Dec 11, 2020·Drug Design, Development and Therapy·Emad H M HassaneinAyman M Mahmoud
Apr 25, 2021·Phytotherapy Research : PTR·Abu Mohammad SyedBidya Dhar Sahu
Aug 28, 2021·Experimental and Therapeutic Medicine·Tengqi LiYiyang Dong

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
PCR
nuclear translocation

Software Mentioned

SPSS PASW Statistics

Related Concepts

Related Feeds

Cardiovascular Disease Pathophysiology

Cardiovascular disease involves several different processes that contribute to the pathological mechanism, including hyperglycemia, inflammation, atherosclerosis, hypertension and more. Vasculature stability plays a critical role in the development of the disease. Discover the latest research on cardiovascular disease pathophysiology here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis