Brazilin Ameliorates Diabetic Nephropathy and Inflammation in db/db Mice

Inflammation
Zhan-Yuan LiHan-Yang Ye

Abstract

Hyperglycemia and inflammation play important roles in the pathogenesis of diabetic nephropathy (DN). Brazilin might be an effective pharmacological agent against hyperglycemia and inflammation. In our present study, we explored whether brazilin mitigated pathological progression, inflammation, and extracellular matrix (ECM) accumulation in a mouse model of diabetic nephropathy. Brazilin reduced aggravated biochemical indices of DN (proteinuria and the serum glucose level) and renal hypertrophy. Brazilin also improved renal morphology and inhibited macrophage infiltration, as manifested by different pathological staining methods. Brazilin reduced the levels of pro-inflammatory cytokines and CD68, a macrophage marker, in the kidney cortex, as revealed by both RT-PCR and western blotting experiments. Furthermore, brazilin significantly downregulated the serum levels of pro-inflammatory cytokines and chemokines. Interestingly, brazilin significantly upregulated the levels of the anti-inflammatory factor IL-10, and prevented ECM accumulation. Brazilin reduced nuclear translocation of the NF-κB p65 subunit both in vitro and in vivo. Thus, brazilin might be a useful treatment for DN, through mitigating hypoglycemia, inflammation, and...Continue Reading

References

Nov 26, 1999·Biochemical Pharmacology·L Y KhilC K Moon
Jul 31, 2004·Journal of the American Society of Nephrology : JASN·Fiona T H LeeJosephine M Forbes
Sep 17, 2004·Planta medica·Hyeon-Soon WonChang-Kiu Moon
May 6, 2005·Journal of the American Society of Nephrology : JASN·Katherine R Tuttle
Jul 5, 2005·Journal of Ethnopharmacology·Eun-Ju YouChang-Kiu Moon
Mar 3, 2009·Cytokine & Growth Factor Reviews·Juan F Navarro-GonzálezJavier García
May 4, 2011·Nature Reviews. Nephrology·Juan F Navarro-GonzálezJavier García-Pérez
Oct 4, 2011·Journal of Ethnopharmacology·Washington Soares Ferreira JúniorUlysses Paulino de Albuquerque
Oct 19, 2012·Clinical Science·Jun Wada, Hirofumi Makino
Jun 1, 2013·Current Opinion in Pharmacology·Fionnuala B Hickey, Finian Martin
Aug 30, 2013·American Journal of Physiology. Renal Physiology·Timothy SullivanJuan C Jaen
Jun 28, 2014·Journal of the American Society of Nephrology : JASN·Juan F Navarro-GonzálezJavier García
Aug 27, 2014·Chinese Journal of Natural Medicines·Qing ZhangQiang Yu
Mar 12, 2015·Phytotherapy Research : PTR·Supinya TewtrakulOrapun Yodsaoue
Jul 16, 2016·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Celine Q F KlessensDaphne H T IJpelaar
Aug 26, 2016·American Journal of Physiology. Renal Physiology·Raimund PichlerKatherine R Tuttle
Jan 5, 2017·The Korean Journal of Internal Medicine·Yaeni Kim, Cheol Whee Park

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
urine collection
ELISA
reverse transcription PCR
nuclear translocation

Software Mentioned

Nikon NIS Elements AR

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here