BRCA1 protein is linked to the RNA polymerase II holoenzyme complex via RNA helicase A

Nature Genetics
S F AndersonJ D Parvin

Abstract

The breast cancer specific tumour suppressor protein, BRCA1 (refs 1,2), activates transcription when linked with a DNA-binding domain and is a component of the RNA polymerase II (Pol II) holoenzyme. We show here that RNA helicase A (RHA) protein links BRCA1 to the holoenzyme complex. The region of BRCA1 which interacts with RHA and, thus, the holoenzyme complex, corresponds to subregions of the BRCT domain of BRCA1 (ref. 9). This interaction was shown to occur in yeast nuclei, and expression in human cells of a truncated RHA molecule which retains binding to BRCA1 inhibited transcriptional activation mediated by the BRCA1 carboxy terminus. These data are the first to identify a specific protein interaction with the BRCA1 C-terminal domain and are consistent with the model that BRCA1 functions as a transcriptional coactivator.

References

Jan 1, 1995·Methods in Enzymology·P Aspenström, M F Olson
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·J P TassanE A Nigg
Oct 7, 1994·Science·P A FutrealY Miki
Jan 1, 1995·Human Molecular Genetics·C I Szabo, M C King
Jul 1, 1996·Nature Genetics·E V KooninP Bork
Aug 22, 1996·Nature·M S Chapman, I M Verma
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·A N MonteiroH Hanafusa
Apr 25, 1997·The Journal of Biological Chemistry·S Zhang, F Grosse
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·R ScullyJ D Parvin
Mar 14, 1998·Nucleic Acids Research·A S NeishJ D Parvin
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·T OuchiH Hanafusa

❮ Previous
Next ❯

Citations

Oct 21, 1999·Journal of Cellular Physiology·Y ChenH K Chew
Aug 30, 2000·International Journal of Cancer. Journal International Du Cancer·K YoshikawaR Takahashi
Mar 10, 2001·Biochemical and Biophysical Research Communications·K ZhuH Busch
Jun 8, 2001·Biochemical and Biophysical Research Communications·G C ChenZ Y Wang
Sep 1, 2012·Molecules and Cells·Suhwan Chang, Shyam K Sharan
Dec 11, 2008·Breast Cancer Research and Treatment·Christopher A PettigrewMelissa A Brown
Sep 6, 2002·Biochimica Et Biophysica Acta·Averell Gnatt
Jul 31, 2010·Biochemistry·Aklank JainKaren M Vasquez
Apr 25, 2006·Nature Reviews. Cancer·Irmgard Irminger-Finger, Charles Edward Jefford
Dec 24, 2009·Nature Reviews. Molecular Cell Biology·Michael S Y HuenJunjie Chen
Mar 24, 2011·Nature Reviews. Molecular Cell Biology·Armen ParsyanNahum Sonenberg
May 9, 2006·Nature Structural & Molecular Biology·Tiffiney Roberts HartmanKathleen Boris-Lawrie
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·B P SchlegelJ D Parvin
Mar 21, 2001·EMBO Reports·G NadeauL Gaudreau
Nov 3, 1998·The EMBO Journal·X ZhangP S Freemont
Aug 14, 2003·Human Molecular Genetics·Peter Hohenstein, Riccardo Fodde
Nov 18, 2004·Journal of the National Cancer Institute·Richard D KennedyD Paul Harkin
Nov 15, 2007·Journal of the National Cancer Institute·Alison M HoseyD Paul Harkin
Apr 25, 2003·Nucleic Acids Research·Kai ZhouChee-Gun Lee
Aug 18, 2006·Nucleic Acids Research·Tuuli VälinevaOlli Silvennoinen
Sep 30, 2008·Nucleic Acids Research·Seung-Won ParkChee-Gun Lee
Apr 14, 2010·Nucleic Acids Research·Prasun Chakraborty, Frank Grosse
Sep 2, 2011·Nucleic Acids Research·Julia J GorskiD Paul Harkin
Sep 28, 2012·Journal of Virology·Li XingLawrence Kleiman
Jun 21, 2001·Molecular and Cellular Biology·S ArataniT Nakajima
Jul 30, 2004·Molecular and Cellular Biology·Annie MoisanLuc Gaudreau
Sep 16, 2004·Molecular and Cellular Biology·Lea M StaritaJeffrey D Parvin
Sep 17, 2005·Molecular and Cellular Biology·Satish SankaranJeffrey D Parvin
Feb 4, 2005·Journal of Medical Genetics·C M PhelanA N A Monteiro
Jan 28, 2010·Cancer Research·Ekaterina P LamberJeffrey D Parvin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Breast Cancer: BRCA1 & BRCA2

Mutations involving BRCA1, found on chromosome 17, and BRCA2, found on chromosome 13, increase the risk for specific cancers, such as breast cancer. Discover the last research on breast cancer BRCA1 and BRCA2 here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
R ScullyJ D Parvin
Proceedings of the National Academy of Sciences of the United States of America
T OuchiH Hanafusa
© 2022 Meta ULC. All rights reserved