BRCA1 regulates PIG3-mediated apoptosis in a p53-dependent manner

Oncotarget
Wenwen ZhangXiaoxiang Guan

Abstract

BRCA1 plays a key role in the regulation of p53-dependent target gene transcription activation. Meanwhile, the p53 inducible gene 3 (PIG3) is a downstream target of p53 and is involved in p53-initiated apoptosis. However, little is known about whether BRCA1 can regulate PIG3-mediated apoptosis. Using a tissue microarray containing 149 breast cancer patient samples, we found that BRCA1 and PIG3 expression status were significantly positively correlated (r = 0.678, P < 0.001) and identified a significant positive correlation between high expression of BRCA1 and/or PIG3 and overall survival (OS). Moreover, we reveal that overexpression of BRCA1 significantly increased expression of PIG3 in cells with intact p53, whereas no increase in PIG3 was observed in p53-null MDA-MB-157 cells and p53-depleted HCT116p53-/- cells. Meanwhile, ectopic expression of BRCA1 could not lead to an increase expression level of prohibitin (PHB), which we have previously identified to induce PIG3-mediated apoptosis. Finally, ChIP analysis revealed that PHB can bind to the PIG3 promoter and activate PIG3 transcription independent of p53, although p53 presence did enhance this process. Taken together, our findings suggest that BRCA1 regulates PIG3-mediated ...Continue Reading

References

Aug 22, 1996·Nature·M S Chapman, I M Verma
Sep 26, 1997·Nature·K PolyakB Vogelstein
Dec 22, 1999·Oncogene·K SomasundaramW S el-Deiry
Mar 29, 2002·Nature Genetics·Ana ContenteMatthias Dobbelstein
May 25, 2002·Molecular and Cellular Biology·Timothy K MacLachlanWafik S El-Deiry
Sep 23, 2003·The Journal of Biological Chemistry·Gina FusaroSrikumar Chellappan
Apr 13, 2005·Trends in Molecular Medicine·Suresh MishraLiam J Murphy
Oct 29, 2005·Molecular Cancer Research : MCR·Junran Zhang, Simon N Powell
Dec 2, 2005·The Journal of Biological Chemistry·Shipra RastogiSrikumar Chellappan
Jan 4, 2008·The Journal of Biological Chemistry·Longchuan ChenXiaohua Wu
Dec 24, 2009·Nature Reviews. Molecular Cell Biology·Michael S Y HuenJunjie Chen
Oct 4, 2011·Molecular Cell·Shailja PathaniaDavid M Livingston
Mar 31, 2012·The Journal of Biological Chemistry·Hui WangYan P Yu
Aug 25, 2012·Cell Death and Differentiation·M Y KangH J You
Jan 7, 2014·Biochemical and Biophysical Research Communications·Xiaoxiang GuanQingyi Wei
Jan 17, 2015·Oncoscience·Joao Paulo Oliveira-CostaFernando Augusto Soares

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Citations

Mar 6, 2017·Journal of Experimental & Clinical Cancer Research : CR·Ming LiZeng-Fu Shang
Sep 14, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Wenwen ZhangXiaoxiang Guan
Mar 30, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jishu QuanMing Jin
Sep 25, 2017·Cell Death and Differentiation·Cédric CastrogiovanniPatrick Dumont
Jan 1, 2018·Cell Death and Differentiation·Cédric CastrogiovanniPatrick Dumont
Apr 24, 2021·Scientific Reports·Kana MiyaharaKeisuke Miyazawa
Apr 2, 2018·Neoplasia : an International Journal for Oncology Research·Juan JinXiaoxiang Guan

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Methods Mentioned

BETA
flow
transfection
fluorescence microscopy
ChIP
immunoprecipitation
PCR

Software Mentioned

SPSS
SPSS Statistics

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