Breakdown of Ribulose Bisphosphate Carboxylase and Change in Proteolytic Activity during Dark-induced Senescence of Wheat Seedlings.

Plant Physiology
V A Wittenbach

Abstract

When 8-day-old wheat seedlings (Triticum aestivum L. var. Chris) are placed in the dark the fully expanded primary leaves undergo the normal changes associated with senescence, for example, loss of chlorophyll, soluble protein, and photosynthetic capacity (Wittenbach 1977 Plant Physiol. 59: 1039-1042). Senescence in this leaf is completely reversible when plants are transferred to the light during the first 2 days, but thereafter it becomes an irreversible process. During the reversible stage of senescence the loss of ribulose bisphosphate carboxylase (RuBPCase) quantitated immunochemically, accounted for 80% of the total loss of soluble protein. There was no significant change in RuBPCase activity per milligram of antibody-recognized carboxylase during this stage despite an apparent decline in specific activity on a milligram of soluble protein basis. With the onset of the irreversible stage of senescence there was a rapid decline in activity per milligram of carboxylase, suggesting a loss of active sites. There was no increase in total proteolytic activity during the reversible stage of senescence despite the loss of carboxylase, indicating that this initial loss was not due to an increase in total activity. An 80% increase i...Continue Reading

References

Sep 1, 1974·Archives of Biochemistry and Biophysics·W BottomleyP R Whitfeld
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Dec 24, 1970·Biochemical and Biophysical Research Communications·N Kawashima, S G Wildman
Jun 1, 1977·Plant Physiology·V A Wittenbach
Jun 1, 1977·Plant Physiology·R H Drivdahl, K V Thimann

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Citations

May 1, 1981·Plant Physiology·W Lin, V A Wittenbach
Nov 1, 1982·Plant Physiology·V A Wittenbach
Dec 1, 1988·Plant Physiology·C BaysdorferW J Vanderwoude
Jul 13, 2012·Bioscience, Biotechnology, and Biochemistry·Sugunya PitakrattananukoolFumio Takaiwa
Apr 2, 2014·Journal of Experimental Botany·Liliana Avila-OspinaCéline Masclaux-Daubresse
Jan 1, 1990·The New Phytologist·R C Huffaker
May 2, 2009·Seminars in Cell & Developmental Biology·Andrew P HaywardS P Dinesh-Kumar
Sep 20, 2008·Plant Biology·P L GregersenK Krupinska
Nov 26, 2013·Biochimica Et Biophysica Acta·Hiroyuki IshidaAmane Makino
Oct 11, 2017·Journal of Experimental Botany·Justine BressonUlrike Zentgraf
Oct 10, 2009·Plant Signaling & Behavior·Shinya Wada, Hiroyuki Ishida

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