Breaking the Barrier - Potent Anti-Inflammatory Activity following Efficient Topical Delivery of Etanercept using Thermoresponsive Nanogels

Theranostics
Michael GiulbudagianMarcelo Calderón

Abstract

Topical administration permits targeted, sustained delivery of therapeutics to human skin. Delivery to the skin, however, is typically limited to lipophilic molecules with molecular weight of < 500 Da, capable of crossing the stratum corneum. Nevertheless, there are indications protein delivery may be possible in barrier deficient skin, a condition found in several inflammatory skin diseases such as psoriasis, using novel nanocarrier systems. Water in water thermo-nanoprecipitation; dynamic light scattering; zeta potential measurement; nanoparticle tracking analysis; atomic force microscopy; cryogenic transmission electron microscopy; UV absorption; centrifugal separation membranes; bicinchoninic acid assay; circular dichroism; TNFα binding ELISA; inflammatory skin equivalent construction; human skin biopsies; immunohistochemistry; fluorescence microscopy; western blot; monocyte derived Langerhans cells; ELISA Results: Here, we report the novel synthesis of thermoresponsive nanogels (tNG) and the stable encapsulation of the anti-TNFα fusion protein etanercept (ETR) (~150 kDa) without alteration to its structure, as well as temperature triggered release from the tNGs. Novel tNG synthesis without the use of organic solvents was c...Continue Reading

Citations

Jun 22, 2019·Expert Opinion on Drug Delivery·Ana Cláudia LimaNuno M Neves
Nov 23, 2019·Nanomedicine·Neha TiwariMarcelo Calderón
Nov 16, 2018·Nanomaterials·Paola Sánchez-MorenoHouria Boulaiz
Dec 8, 2020·Biomaterials Science·Doğuş IşıkDaniel Klinger
Jan 10, 2021·Pharmaceutics·Daniela Placha, Josef Jampilek
Dec 18, 2019·Journal of Controlled Release : Official Journal of the Controlled Release Society·Irene Dolz-PérezMaría J Vicent
Sep 3, 2020·Trends in Biotechnology·Qurrat Ul AinSarah Hedtrich
Oct 14, 2018·Advanced Drug Delivery Reviews·Alexandre BordatNicolas Tsapis
Jun 11, 2020·ACS Applied Materials & Interfaces·Ernesto R Osorio-BlancoMarcelo Calderón
Sep 7, 2021·Angewandte Chemie·Neha TiwariMarcelo Calderón
Dec 10, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Felix ReisbeckRainer Haag

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Methods Mentioned

BETA
nanoprecipitation
dynamic light scattering
transmission electron microscopy
Protein Assay
ELISA
electrophoresis
flow cytometry
AFM
biopsies

Software Mentioned

NanoScope Analysis
FlowJo

Related Concepts

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