Breast adenocarcinoma MCF-7 cell line induces spontaneous osteoclastogenesis via a RANK-ligand-dependent pathway

Acta histochemica
Vanessa NicolinPaola Narducci

Abstract

The metastasis of breast cancer to the skeleton is a serious clinical problem resulting in hypercalcemia, bone fragility and insurmountable pain. The invasion of bony tissue by neoplastic cells usually very rapidly affects the balance between bone apposition and bone resorption. In order to elucidate a mechanism for cancer-induced osteoclastogenesis, cells from a human breast cancer line, MCF-7, were directly co-cultured with murine monocytes RAW 264.7 type CRL 2278. Compared with controls, co-culture of MCF-7 induced differentiation of multinucleated cells by membrane-bound and soluble receptor activator of NF-kB ligand (RANKL) as quantified by ELISA, Western blot analysis, transmission electron microscopy (TEM), and immunocytochemistry. The aim of this study was to determine an in vitro model system of MCF-7 human breast cancer cells grown together with monocytes to show that expression of RANKL promotes osteoclastogenesis, which may indicate a mechanism for the development of osteolytic lesions in breast cancer bone metastasis.

References

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Citations

Mar 16, 2013·Clinical & Experimental Metastasis·Jessica M GrundaGregory A Clines
Mar 1, 2013·Journal of the National Cancer Institute·Ashley SutherlandCarrie S Shemanko
Jan 26, 2012·Journal of Cellular Biochemistry·João Costa-RodriguesMaria H Fernandes
Apr 24, 2010·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Yong Qian, Hong-Zhang Huang
Aug 30, 2016·International Journal of Molecular Sciences·Chiara ArrigoniMatteo Moretti
Jun 16, 2011·Molecular Biology Reports·Kai ZhaoPing Wang

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