Breast Cancer Anti-Estrogen Resistance 4 (BCAR4) Drives Proliferation of IPH-926 lobular Carcinoma Cells

PloS One
T van AgthovenMatthias Christgen

Abstract

Most breast cancers depend on estrogenic growth stimulation. Functional genetic screenings in in vitro cell models have identified genes, which override growth suppression induced by anti-estrogenic drugs like tamoxifen. Using that approach, we have previously identified Breast Cancer Anti-Estrogen Resistance 4 (BCAR4) as a mediator of cell proliferation and tamoxifen-resistance. Here, we show high level of expression and function of BCAR4 in human breast cancer. BCAR4 mRNA expression was evaluated by (q)RT-PCR in a panel of human normal tissues, primary breast cancers and cell lines. A new antibody raised against C78-I97 of the putative BCAR4 protein and used for western blot and immunoprecipitation assays. Furthermore, siRNA-mediated gene silencing was implemented to study the function of BCAR4 and its downstream targets ERBB2/3. Except for placenta, all human normal tissues tested were BCAR4-negative. In primary breast cancers, BCAR4 expression was comparatively rare (10%), but associated with enhanced proliferation. Relative high BCAR4 mRNA expression was identified in IPH-926, a cell line derived from an endocrine-resistant lobular breast cancer. Moderate BCAR4 expression was evident in MDA-MB-134 and MDA-MB-453 breast can...Continue Reading

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Oct 8, 2016·Oncotarget·Qin-Nan ChenMing Sun
Sep 14, 2018·International Journal of Molecular Sciences·Alma D Campos-ParraCarlos Pérez-Plasencia
Feb 25, 2019·Breast Cancer Research and Treatment·Emily A BossartSteffi Oesterreich
Jun 17, 2020·Therapeutic Advances in Medical Oncology·Yidi QuDi Wang
Jun 26, 2020·Clinical Genetics·He-da ZhangJin-Hai Tang
Mar 9, 2020·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Wenxiao JiangXueqiong Zhu
May 28, 2021·Human Cell·Qiong YiYayi Xia

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Methods Mentioned

BETA
PCR
immunoprecipitation
transfection

Clinical Trials Mentioned

NCT00225758

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