Breast cancer proteomics by laser capture microdissection, sample pooling, 54-cm IPG IEF, and differential iodine radioisotope detection

Electrophoresis
Hans NeubauerMichael A Cahill

Abstract

The presence of progesterone receptor (PR) in estrogen receptor (ER)-positive breast cancer is associated with a good prognosis, and indicates that tumors are likely to respond to tamoxifen. However, ER+/PR- tumors respond less well. To reveal the potential molecular mechanism of this phenomenon, we sought to identify differential protein abundances between invasive ductal carcinoma cells from cryopreserved ER+/PR+ and ER+/PR- mammary tumor specimens. Because current proteomics methods are hampered in the examination of most primary human tumor samples by the extreme tissue heterogeneity, we used laser capture microdissection (LCM) to isolate tumor cells and developed a sample pooling strategy to analyze small sample protein lysates. Proteins from LCM-harvested tumors were pooled into four sub-pools from each condition of three tumors/sub-pool, and proteins from respective paired sub-pools were co-electrophoresed by 2-DE using 54-cm IEF over pH 4-9. Abundance ratios were accurately quantified by a differential multiplex radioactive ProteoTope method at low attomole levels ( approximately 3.6 microg protein per labeling reaction, <180 ng per multiplex protein sample per 54-cm gel). Applying this approach, differentially displaye...Continue Reading

References

Jan 1, 1994·Methods in Enzymology·J J Mieyal, D W Starke
Mar 15, 1993·Cancer·I C Henderson
Nov 26, 1998·The New England Journal of Medicine·C K Osborne
Mar 6, 1999·Breast Cancer Research and Treatment·C K Osborne
Aug 5, 2000·Endocrinology·W P BocchinfusoK S Korach
Feb 24, 2001·Genome Biology·D Werck-Reichhart, R Feyereisen
May 15, 2001·Endocrine-related Cancer·D C AllredS A Fuqua
Jan 5, 2002·Journal of the National Cancer Institute. Monographs·B FisherN Wolmark
Oct 22, 2002·Lancet·Daniel W Nebert, David W Russell
Dec 14, 2002·Journal of Biochemical and Molecular Toxicology·Todd D Porter
Feb 1, 2003·Pharmacology & Therapeutics·John B Schenkman, Ingela Jansson
Aug 20, 2003·Biostatistics·C M KendziorskiA D Attie
Mar 18, 2004·Molecular and Cellular Endocrinology·Li MinMitshuhiro Okamoto
Apr 15, 2004·Journal of Mammary Gland Biology and Neoplasia·Elizabeth Anderson, Robert B Clarke
Jun 4, 2004·The Journal of Biological Chemistry·Blandine KedjouarMarc Poirot
Jun 16, 2004·Cellular and Molecular Life Sciences : CMLS·A-M Simeone, A M Tari
Oct 13, 2004·Mutation Research·Richard M Niles
Oct 27, 2004·Disease Markers·George M Kunz, Daniel W Chan
Nov 16, 2004·Proteomics·Angelika GörgMichael J Dunn
Dec 15, 2004·Breast Cancer Research and Treatment·Suzanne Aw Fuqua, Yukun Cui
Dec 18, 2004·Journal of Neuroscience Research·Ikuo KimuraNobuyuki Itoh
Jan 18, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Richard I SomiariCraig D Shriver
Jan 22, 2005·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Mar 8, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Renate Burgemeister
May 18, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Timothy W VogelRobert J Weil
Oct 20, 2005·The Journal of Pharmacology and Experimental Therapeutics·Gerard CruddenRolf J Craven

❮ Previous
Next ❯

Citations

Sep 13, 2008·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·M KlemmA Schrattenholz
Sep 23, 2011·Journal of Proteome Research·Shawna Mae HengelDavid R Goodlett
Mar 31, 2009·Molecular & Cellular Proteomics : MCP·Arzu UmarLjiljana Pasa-Tolić
Oct 31, 2006·Photomedicine and Laser Surgery
Aug 10, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Cheng-Jeng TaiYu-Jia Chang
Jun 17, 2009·Journal of Cancer Research and Clinical Oncology·Xiang-Min LiZhi-Qiang Xiao
Jun 6, 2008·Expert Review of Proteomics·Arzu UmarLjiljana Pasa-Tolic
Oct 19, 2007·Expert Review of Proteomics·Howard B Gutstein, Jeffrey S Morris
Jan 26, 2007·Expert Opinion on Biological Therapy·Thomas Y K LauWilliam M Gallagher
Dec 8, 2015·Journal of Proteomics·Monika DvořákováPavel Bouchal
Jul 27, 2010·The Journal of Surgical Research·Marlin Wayne CauseyJonathan D Stallings
Aug 14, 2009·Electrophoresis·Angel P DizDavid O F Skibinski
Jun 12, 2009·Electrophoresis·Elisabetta Gianazza, Pier Giorgio Righetti
Sep 1, 2007·Proteomics. Clinical Applications·Mark Aspinall-O'Dea, Eithne Costello
Mar 14, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Linna WangWenxin Wu
Oct 4, 2011·Journal of Proteomics·Xin-Hui LiZhi-Qiang Xiao
Apr 5, 2007·Molecular Cancer·William C S Cho
Oct 26, 2013·Medical Oncology·Keivan Majidzadeh-A, Javad Gharechahi
May 20, 2014·Expert Review of Proteomics·Pavel GromovIrina Gromova
Apr 12, 2020·Molecular Phylogenetics and Evolution·Elisabeth HehenbergerMichael A Cahill
Oct 22, 2008·Journal of Proteome Research·Mónica Martínez-FernándezEmilio Rolán-Alvarez
Mar 4, 2008·Journal of Proteome Research·Hubert HondermarckXuefen Le Bourhis
Dec 2, 2006·Journal of Proteome Research·Vukic SoskicAndre Schrattenholz

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Metabolism

In order for cancer cells to maintain rapid, uncontrolled cell proliferation, they must acquire a source of energy. Cancer cells acquire metabolic energy from their surrounding environment and utilize the host cell nutrients to do so. Here is the latest research on cancer metabolism.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.