Breast fibroblasts in both cancer and normal tissues induce phenotypic transformation of breast cancer stem cells: a preliminary study

PeerJ
Bixiao WangHua Kang

Abstract

Breast cancer stem cells (BCSCs) are associated with the invasion of breast cancer. In recent years, studies have demonstrated different phenotypes among BCSCs. Furthermore, BCSCs of diverse phenotypes are present at different tumour sites and different histological stages. Fibroblasts are involved in the phenotypic transformation of BCSCs. Cancer-associated fibroblasts (CAFs) participate in the induction of epithelial-mesenchymal transition, thereby promoting the acquisition of stem cell characteristics, but little is known about the role of normal fibroblasts (NFs) in the phenotypic transformation of BCSCs or about the effect of CAFs and NFs on BCSC phenotypes. A total of six pairs of primary CAFs and NFs were isolated from surgical samples of breast cancer patients and subjected to morphological, immunohistochemical, cell invasion and proteomics analyses. After establishing a cell culture system with conditioned medium from CAFs and NFs, we used the mammosphere formation assay to explore the effect of CAFs and NFs on the self-renewal ability of BCSCs. The effect of CAFs and NFs on the phenotypic differentiation of BCSCs was further analysed by flow cytometry and immunofluorescence. The isolated CAFs and NFs did not show sign...Continue Reading

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Citations

Nov 30, 2019·Analytical Cellular Pathology (Amsterdam)·Monica NeaguMarieta Costache
Jun 14, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Z ChenH Kang
Jan 6, 2021·Journal of Mammary Gland Biology and Neoplasia·María Belén GiorelloNorma Alejandra Chasseing
Jan 20, 2021·ACS Biomaterials Science & Engineering·Tamaghna GuptaIshwar K Puri
Sep 23, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ali Akbar SamadaniMagtouf Gatei

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Methods Mentioned

BETA
surgical resection
flow cytometry
fluorescence activated cell-sorting
FACS
FCS

Software Mentioned

Firmiana

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