Breed, Diet, and Interaction Effects on Adipose Tissue Transcriptome in Iberian and Duroc Pigs Fed Different Energy Sources

Genes
R BenítezC Óvilo

Abstract

In this study, we analyzed the effects of breed, diet energy source, and their interaction on adipose tissue transcriptome in growing Iberian and Duroc pigs. The study comprised 29 Iberian and 19 Duroc males, which were kept under identical management conditions except the nutritional treatment. Two isoenergetic diets were used with 6% high oleic sunflower oil (HO) or carbohydrates (CH) as energy sources. All animals were slaughtered after 47 days of treatment at an average live weight of 51.2 kg. Twelve animals from each breed (six fed each diet) were employed for ham subcutaneous adipose tissue RNA-Seq analysis. The data analysis was performed using two different bioinformatic pipelines. We detected 837 and 1456 differentially expressed genes (DEGs) according to breed, depending on the pipeline. Due to the strong effect of breed on transcriptome, the effect of the diet was separately evaluated in the two breeds. We identified 207 and 57 DEGs depending on diet in Iberian and Duroc pigs, respectively. A joint analysis of both effects allowed the detection of some breed-diet interactions on transcriptome, which were inferred from RNA-Seq and quantitative PCR data. The functional analysis showed the enrichment of functions relate...Continue Reading

References

Feb 27, 1999·Current Opinion in Cell Biology·A R Bresnick
Oct 31, 2000·Nutrition·P Björntorp, R Rosmond
Sep 20, 2001·Biomaterials·N KanzakiA Ito
Feb 14, 2002·The Journal of Investigative Dermatology·Franck Verrecchia, Alain Mauviel
Jul 2, 2002·Journal of Lipid Research·Shubha MurthyF Jeffrey Field
Sep 6, 2002·Biochemical Pharmacology·Giuseppe FilomeniMaria Rosa Ciriolo
Apr 3, 2003·Nature Reviews. Genetics·Michael Müller, Sander Kersten
Oct 24, 2003·Nature·Anne-Sophie Van LaereLeif Andersson
Jan 24, 2004·Biochimie·Richard W Hanson, Lea Reshef
Feb 26, 2004·General and Comparative Endocrinology·Ruqian ZhaoRoland Grossmann
Dec 3, 2005·Nature Reviews. Immunology·Siamon Gordon, Philip R Taylor
Dec 6, 2005·La Presse médicale·S Boullu-CioccaC Oliver
Mar 3, 2006·The Journal of Clinical Investigation·Noam Zelcer, Peter Tontonoz
Jul 11, 2006·BMC Bioinformatics·Mathieu MironRobert Nadon
Oct 6, 2006·Obesity·Rexford S Ahima
Apr 28, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shaikh Mizanoor RahmanJacob E Friedman
Sep 8, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Birgit GustafsonUlf Smith
Oct 17, 2007·Annual Review of Physiology·Martin G MyersHeike Münzberg
Feb 12, 2008·Nature Genetics·Gilles ThomasStephen J Chanock
Jun 21, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Caroline M ApovianNoyan Gokce
Feb 5, 2009·Current Diabetes Reports·Dario A GutierrezAlyssa H Hasty
Feb 26, 2009·BMC Genomics·Miguel Pérez-EncisoManel López-Béjar
Dec 9, 2009·Biochemical and Biophysical Research Communications·Le Thi Kim ChungMakoto Funaki
Jan 29, 2010·Cellular and Molecular Life Sciences : CMLS·Edwin C M Mariman, Ping Wang
May 11, 2010·Journal of Applied Genetics·M SwitonskiM Grzes
Jun 29, 2010·Metabolism: Clinical and Experimental·Ling LiHaihong Zong
Nov 6, 2010·Antioxidants & Redox Signaling·Dongping Liu, Yang Xu
Nov 20, 2010·Bioinformatics·Timo LassmannCarsten O Daub
Feb 23, 2011·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·A OlivaresC J López-Bote
Aug 26, 2011·Metabolism: Clinical and Experimental·Ashley J PeckettMichael C Riddell
Sep 13, 2011·Meat Science·Carmen BurgosPascual López-Buesa
Oct 28, 2011·Meat Science·Carolina Pugliese, Francesco Sirtori
Jan 19, 2012·International Journal of Biological Sciences·Gangwen HanXiao-Jing Wang

❮ Previous
Next ❯

Citations

Nov 2, 2019·Journal of Applied Genetics·Joanna Nowacka-Woszuk
Mar 7, 2020·Animals : an Open Access Journal From MDPI·Klavdija PoklukarMartin Škrlep
Feb 14, 2021·International Journal of Molecular Sciences·Klavdija PoklukarMartin Škrlep

❮ Previous
Next ❯

Methods Mentioned

BETA
RNA-Seq
PCR

Software Mentioned

Cufflinks
Genorm
TopHat
Ingenuity Pathway Analysis ( IPA
ENSEMBL
HTSeq
IPA Canonical Pathways Analysis
Qualimap
FastQC
Cuffdiff

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.