Breeding of D(-)-lactic acid high producing strain by low-energy ion implantation and preliminary analysis of related metabolism

Applied Biochemistry and Biotechnology
Tingting XuBingfang He

Abstract

The low-energy nitrogen ion beam implantation technique was used in the breeding of mutant D(-)-lactic-acid-producing strains. The wild strain Sporolactobacillus sp. DX12 was mutated by an N(+) ion beam with energy of 10keV and doses ranging from 0.4 x 10(15) to 6.60 x 10(15) ions/cm(2). Combined with an efficient screening method, an efficient mutant Y2-8 was selected after two times N(+) ion beam implantation. By using the mutant Y2-8, 121.6g/l of D-lactic acid was produced with the molar yields of 162.1% to the glucose. The yield of D-lactic acid by strain Y2-8 was 198.8% higher than the wild strain. Determination of anaerobic metabolism by Biolog MT2 was used to analyze the activities of the concerned enzymes in the lactic acid metabolic pathway. The results showed that the activities of the key enzymes responded on the substrates such as 6-phosphofructokinase, pyruvate kinase, and D-lactate dehydrogenase were considerably higher in the mutants than the wild strain. These might be affected by ion beam implantation.

References

Feb 6, 2004·Trends in Biotechnology·Willem M de Vos, Jeroen Hugenholtz
May 7, 2004·Nature·Markus W CovertBernhard O Palsson
Jun 1, 2004·The Journal of Biological Chemistry·Byoung-Mo KooYeong-Jae Seok
Sep 21, 2005·Bioresource Technology·Takaaki TanakaMasayuki Taniguchi
Oct 20, 2005·Journal of Bioscience and Bioengineering·Shigenobu MiuraMitsuyasu Okabe
Mar 11, 2006·Applied Microbiology and Biotechnology·Shao-Bin GuZeng-Liang Yu
Mar 30, 2006·Journal of Bioscience and Bioengineering·Nobuhiro IshidaHaruo Takahashi
Aug 10, 2006·Journal of Industrial Microbiology & Biotechnology·Caixin SuZengliang Yu
Jan 10, 2008·Applied Microbiology and Biotechnology·Shao-Bin GuZeng-Liang Yu
Jan 12, 2008·Applied Microbiology and Biotechnology·Shohei OkinoHideaki Yukawa

❮ Previous
Next ❯

Citations

Jun 2, 2012·Indian Journal of Microbiology·Li ShichangWang Dongdong
Jun 8, 2013·Preparative Biochemistry & Biotechnology·Weidong QianChanglong Cai
Jul 27, 2011·Journal of Industrial Microbiology & Biotechnology·Ting GuoPing-Kai Ouyang
Aug 16, 2012·Journal of Industrial Microbiology & Biotechnology·Lu ZhengBingfang He
Oct 21, 2016·Applied Microbiology and Biotechnology·Silvia KlotzUlf Prüße
Jul 17, 2012·Applied Biochemistry and Biotechnology·Dong LiuHanjie Ying
May 20, 2021·Bioprocess and Biosystems Engineering·Hefang WuZhiming Zheng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Epigenetics Insights from Twin Studies

Find the latest research on epigenetics and twin studies here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.