PMID: 9658088Jul 11, 1998Paper

Brefeldin A inhibits cell-free, de novo synthesis of poliovirus

Journal of Virology
A CuconatiE Wimmer

Abstract

Brefeldin A (BFA), an inhibitor of intracellular vesicle-dependent secretory transport, is a potent inhibitor of poliovirus RNA replication in infected cells. We have determined that the unknown mechanism of BFA inhibition of replication is reproduced in the cell-free poliovirus translation, replication, and encapsidation system. Furthermore, we provide evidence suggesting that the cellular mechanism targeted by BFA, the GTP-dependent synthesis of secretory transport vesicles, may be involved in viral RNA replication in the system via a soluble cellular GTP-binding and -hydrolyzing activity. This activity is related to the ARF (ADP-ribosylation factor) family of GTP-binding proteins. ARFs are required for the formation of several classes of secretory vesicles, and some family members are indirectly inactivated by BFA. Peptides that function as competitive inhibitors of ARF activity in cell-free transport systems also inhibit poliovirus RNA replication, and this inhibitory effect can be countered by the addition of exogenous ARF. We suggest that BFA inhibition of replication is diagnostic of a requirement for ARF activity in the cell-free system.

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Citations

Feb 1, 2013·Journal of Virology·Minetaro AritaHiroyuki Shimizu
Jun 13, 2014·Journal of Virology·George A Belov, Elizabeth Sztul
Jul 11, 2014·Journal of Virology·Lauren A Ford SiltzGeorge A Belov
May 14, 2005·Journal of Virology·George A BelovEllie Ehrenfeld
Apr 14, 2005·Journal of Virology·Julie R Harris, Vincent R Racaniello
Jul 28, 2010·The Journal of General Virology·Minetaro AritaHiroyuki Shimizu
Dec 11, 2007·DNA and Cell Biology·Nickolay NeznanovAndrei V Gudkov
Oct 26, 2012·Journal of Virology·Kiwamu HyodoTetsuro Okuno
Aug 7, 2002·Journal of Virology·C RitzenthalerP Pfeiffer
Apr 17, 2008·Nature Reviews. Microbiology·Sven Miller, Jacomine Krijnse-Locker
Mar 26, 2003·Annual Review of Phytopathology·Amine O Noueiry, Paul Ahlquist
Aug 10, 2007·Journal of Virology·Jason D GraciCraig E Cameron
Jan 8, 2013·Future Virology·Amanda J Chase, Bert L Semler
Jun 15, 2007·Journal of Virology·George A BelovEllie Ehrenfeld
Nov 3, 2006·Journal of Virology·George A BelovEllie Ehrenfeld
Sep 30, 2005·Traffic·Jason Mackenzie
Nov 3, 2017·Tropical Medicine and Health·Muhareva RaekiansyahKouichi Morita
Apr 4, 2021·Viruses·Terry Jackson, Graham J Belsham
May 22, 2004·The Journal of Organic Chemistry·Yikang WuJia-Hui Huang

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