Brefeldin A inhibits cholesterol efflux without affecting the rate of cellular uptake and re-secretion of apolipoprotein A-I in adipocytes.

Archives of Biochemistry and Biophysics
Philip B VergheseJose L Soulages

Abstract

A possible role of cellular uptake and re-secretion of apoA-I in the mechanism of cholesterol efflux induced by apoA-I was investigated using a novel experimental approach. Incubation of adipocytes with a recombinant human apoA-I containing a consensus PKA phosphorylation site, pka-ApoA-I, leads to the appearance of phosphorylated protein in the cell culture medium unambiguously proving cellular uptake and re-secretion of pka-ApoA-I. Phosphorylation of apoA-I is abolished by PKA inhibitors and enhanced by PKA activators demonstrating the specific involvement of PKA. Studies on the concentration dependence of pka-apoA-I phosphorylation and competition experiments with human apoA-I suggest that apolipoprotein uptake is a receptor mediated process. A possible role of apoA-I recycling in the mechanism of cholesterol efflux was investigated by determining the rates of apoA-I induced cholesterol efflux and apoA-I recycling in the presence and in the absence of Brefeldin A (BFA). The studies showed that BFA strongly inhibits cholesterol efflux without affecting the rate of apoA-I recycling. Since BFA affects vesicular trafficking of ABCA1, this study suggests that the interaction of apoA-I with ABCA1 does not mediate apolipoprotein up...Continue Reading

References

Oct 1, 1992·The Journal of Cell Biology·S A Wood, W J Brown
Jan 1, 1992·Annual Review of Nutrition·G AilhaudR Négrel
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y Takahashi, J D Smith
Apr 12, 2000·Biochemistry·M P HaynesG H Rothblat
Jan 21, 2001·The Journal of Biological Chemistry·O ChambenoitG Chimini
Apr 20, 2001·The Journal of Biological Chemistry·N WangA R Tall
May 12, 2001·The Journal of Biological Chemistry·E B NeufeldH B Brewer
Mar 15, 2002·The Journal of Biological Chemistry·Jonathan D SmithPing Zheng
Mar 5, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·John F Oram
May 10, 2003·Arteriosclerosis, Thrombosis, and Vascular Biology·Nan Wang, Alan R Tall
May 20, 2003·Journal of Lipid Research·Soazig Le LayIsabelle Dugail
Jan 3, 2004·Journal of Lipid Research·Jonathan D SmithMichael N Oda
Apr 15, 2005·The Journal of Biological Chemistry·Rajesh T PatelEstela L Arrese
Nov 15, 2005·Arteriosclerosis, Thrombosis, and Vascular Biology·Shinji Yokoyama
Mar 29, 2007·Molecular and Cellular Biochemistry·Philip B VergheseJose L Soulages
May 5, 2007·Arteriosclerosis, Thrombosis, and Vascular Biology·Charulatha VedhachalamMichael C Phillips

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Citations

Jun 11, 2010·Molecular and Cellular Biochemistry·Alisha D HowardJose L Soulages
Apr 2, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Nicholas N LyssenkoMichael C Phillips
Jul 21, 2010·Lipids in Health and Disease·Karima BencharifRégis Roche
Dec 23, 2009·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·B-L YuJ-R Hu
Aug 7, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Karin KornmuellerRuth Prassl
Jan 1, 2020·Journal of the American Heart Association·Bikash ManandharKerry-Anne Rye
Feb 26, 2016·Lipids in Health and Disease·Shuai WangYuhong Yi

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