Brefeldin A provokes indirect activation of cdc2 kinase (MPF) in Xenopus oocytes, resulting in meiotic cell division

Developmental Biology
O Mulner-LorillonG Schmalzing

Abstract

Brefeldin A, a fungal metabolite which disrupts protein traffic, provokes indirect activation of cdc2 protein kinase in Xenopus oocytes. Cdc2 protein kinase activation was judged by MPF (M-phase factor) transfer activity, histone H1 kinase activity, and phosphorylation in vivo of the guanine-nucleotide exchange complex EF-1 beta gamma delta. Oocytes resumed complete meiosis upon brefeldin A treatment. Cdc2 protein kinase, MAP kinase, cyclin B, MPF, and protein synthesis changes were all comparable in brefeldin A-treated oocytes and in progesterone-induced oocytes. ED50 for brefeldin A was 0.6 microM. Brefeldin A activation of cdc2 protein kinase occurs with a long time course. Simultaneous treatment of the oocytes at a subthreshold concentration of 1 nM progesterone and 30 microM brefeldin A considerably shortened the kinetics of maturation. Brefeldin A induction of maturation was sensitive to drugs that act on cAMP metabolism. ID50 for IBMX was 0.1 mM, compared to 1 mM for progesterone-treated oocytes. Brefeldin A inhibited protein traffic in oocytes as determined from protein export experiments. ID50 was between 0.1 and 1 microM. Our results give new insights into the possible mechanism of induction of meiotic maturation and ...Continue Reading

Citations

May 15, 2002·Bioscience, Biotechnology, and Biochemistry·Katsuyoshi KamiieShin-ichiro Ejiri
Nov 12, 2013·Reproduction, Fertility, and Development·Lian-Jun ZhangWei Shen
Sep 10, 2002·American Journal of Physiology. Renal Physiology·Wei-Zhong ZengChou-Long Huang
Dec 26, 2000·The Journal of Biological Chemistry·M SchwakeT J Jentsch
May 13, 2020·Cells·Catherine JessusEvelyn Houliston
Nov 5, 1997·The Journal of Biological Chemistry·R A ShimketsC M Canessa
Aug 19, 2007·Development·Wassim El-JouniKhaled Machaca

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