Abstract
Brevetoxins, the algal toxins produced by Karenia brevis during red tide blooms, adversely impact health following ingestion or inhalation. Inhalation of brevetoxins results in a variety of acute symptoms including coughing, wheezing, and shortness of breath. Analysis of manatee lungs following death by purported brevetoxicosis has identified brevetoxin accumulation within macrophages, with pathological manifestions of lung congestion, inflammation, and edema. The goals of this work were to specifically examine effects of brevetoxin-2 on alveolar macrophages, a key cell in responding to toxins in the lung, as well as to determine if brevetoxin-2 results in an inflammatory response and/or direct cytotoxicity. Exposure of an alveolar macrophage cell line (MH-S) to an environmentally and physiologically relevant dose of brevetoxin-2 (0.5microg/ml) did not significantly alter cellular growth over a 24h time period. However, exposure of MH-S cells to brevetoxin-2 for 6h increased phagocytosis of latex beads, increased secretion of interleukin (IL)-2, IL-4, and tumor necrosis factor-alpha, and decreased secretion of IL-5. Very few changes were seen in gene expression following 3 or 6h exposure to brevetoxin-2. These results show that...Continue Reading
References
Apr 21, 1998·Toxicologic Pathology·G D BossartS D Wright
Jun 8, 1999·Annual Review of Immunology·A Aderem, D M Underhill
Mar 4, 2000·Environmental Health Perspectives·F M Van Dolah
Feb 7, 2002·Genomics·Purvesh KhatriStephen A Krawetz
Dec 13, 2002·Bulletin of Environmental Contamination and Toxicology·R H PierceY Zhou
Jan 1, 2003·Nature·Hong WangKevin J Tracey
Jun 10, 2003·Journal of Applied Toxicology : JAT·Brian M Paddle
Oct 8, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Patrick J WalshDaniel G Baden
Jun 24, 2004·Biology of the Neonate·Jo Rae Wright
Sep 17, 2004·Journal of Toxicology and Environmental Health. Part a·Janet BensonDaniel Baden
Sep 28, 2004·American Journal of Respiratory and Critical Care Medicine·William M AbrahamDaniel G Baden
Jan 5, 2005·Nature Reviews. Immunology·Jo Rae Wright
May 4, 2005·Environmental Health Perspectives·Janet M BensonDaniel G Baden
May 4, 2005·Environmental Health Perspectives·William M AbrahamDaniel G Baden
May 4, 2005·Environmental Health Perspectives·Yung Sung ChengDan G Baden
May 4, 2005·Environmental Health Perspectives·Lora E FlemingDaniel G Baden
Jun 16, 2005·Environmental Science & Technology·Yung Sung ChengDaniel G Baden
Mar 9, 2006·Bioinformatics·Lee WengDouglas E Bassett
Jan 16, 2007·Chest·Lora E FlemingDaniel G Baden
Feb 3, 2007·The Journal of Clinical Investigation·Kevin J Tracey
Dec 29, 2007·The Journal of Pathology·X Zhang, D M Mosser
Sep 6, 2008·Clinical and Experimental Immunology·G Passalacqua, G Ciprandi
Aug 30, 2008·Marine Drugs·Catherine J WalshJohn W Sleasman
Sep 2, 2008·Ecotoxicology·R H Pierce, M S Henry
Oct 1, 2006·Harmful Algae·Barbara KirkpatrickDaniel G Baden
Apr 1, 2004·Harmful Algae·Barbara KirkpatrickDaniel G Baden
Citations
Sep 6, 2012·Drug and Chemical Toxicology·Misagh AlipourZacharias E Suntres
Dec 21, 2017·Marine Drugs·Joana AssunçãoF Xavier Malcata
Sep 22, 2018·Journal of Phycology·Benjamin J KramerAlison R Taylor
Sep 24, 2014·Quality of Life Research : an International Journal of Quality of Life Aspects of Treatment, Care and Rehabilitation·Sun SunKristina Burström
Jan 24, 2018·Toxicon : Official Journal of the International Society on Toxinology·Ophelie PierreRaphaele Le Garrec