BRF1, a subunit of RNA polymerase III transcription factor TFIIIB, is essential for cell growth of Trypanosoma brucei

Parasitology
D E Vélez-RamírezS Martínez-Calvillo

Abstract

RNA polymerase III (Pol III) synthesizes small RNA molecules that are essential for cell viability. Accurate initiation of transcription by Pol III requires general transcription factor TFIIIB, which is composed of three subunits: TFIIB-related factor BRF1, TATA-binding protein and BDP1. Here we report the molecular characterization of BRF1 in Trypanosoma brucei (TbBRF1), a parasitic protozoa that shows distinctive transcription characteristics. In silico analysis allowed the detection in TbBRF1 of the three conserved domains located in the N-terminal region of all BRF1 orthologues, namely a zinc ribbon motif and two cyclin repeats. Homology modelling suggested that, similarly to other BRF1 and TFIIB proteins, the TbBRF1 cyclin repeats show the characteristic structure of five α-helices per repeat, connected by a short random-coiled linker. As expected for a transcription factor, TbBRF1 was localized in the nucleus. Knock-down of TbBRF1 by RNA interference (RNAi) showed that this protein is essential for the viability of procyclic forms of T. brucei, since ablation of TbBRF1 led to growth arrest of the parasites. Nuclear run-on and quantitative real-time PCR analyses demonstrated that transcription of all the Pol III-dependent ...Continue Reading

References

Oct 1, 1992·Genes & Development·T Colbert, S Hahn
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G A KassavetisE P Geiduschek
Feb 15, 1993·European Journal of Biochemistry·I M Willis
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S Buratowski, H Zhou
Sep 1, 1997·Molecular and Biochemical Parasitology·V NakaarC Tschudi
Jan 20, 1998·Trends in Biochemical Sciences·M E NobleL N Johnson
Oct 25, 2000·Protein Science : a Publication of the Protein Society·H T ChenR A Scott
Oct 17, 2002·Genes & Development·Laura Schramm, Nouria Hernandez
May 14, 2003·The EMBO Journal·Zoe A Felton-EdkinsPamela H Scott
Mar 22, 2005·International Journal for Parasitology·Silvie Foldynová-TrantírkováJulius Lukes
Jul 16, 2005·Science·Matthew BerrimanNajib M El-Sayed
Sep 17, 2005·Molecular and Cellular Biology·Christine ConesaGiorgio Dieci
Sep 15, 2006·Nucleic Acids Research·Rebecca A Haeusler, David R Engelke
Nov 1, 2006·Biochemical Society Transactions·G A Kassavetis, E P Geiduschek
Jun 5, 2007·Molecular Microbiology·Laurence LecordierLuc Vanhamme
Jul 4, 2007·Molecular and Cellular Biology·Tu N NguyenArthur Günzl
Oct 17, 2007·Cell Cycle·Sarah J Goodfellow, Robert J White
Oct 24, 2007·Eukaryotic Cell·Anish DasVivian Bellofatto
Nov 6, 2007·Trends in Genetics : TIG·Giorgio DieciAldo Pagano
Aug 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·B Syed IbrahimDavid A Wah
Feb 20, 2010·Journal of Biomedicine & Biotechnology·Santiago Martínez-CalvilloElisa E Figueroa-Angulo
May 5, 2011·Nature Reviews. Genetics·Robert J White
Oct 16, 2012·Biochimica Et Biophysica Acta·Joël AckerOlivier Lefebvre
Nov 7, 2012·Biochimica Et Biophysica Acta·Giorgio DieciRoberto Ferrari
Nov 21, 2012·Biochimica Et Biophysica Acta·Robyn D Moir, Ian M Willis
Dec 31, 2014·Eukaryotic Cell·Norma E Padilla-MejíaSantiago Martínez-Calvillo

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Citations

Oct 7, 2016·Parasitology·Rodrigo Moreno-CamposSantiago Martínez-Calvillo
Nov 2, 2016·Molecular Microbiology·Gabriela Romero-MezaSantiago Martínez-Calvillo
May 11, 2019·Cells·Santiago Martínez-CalvilloTomás Nepomuceno-Mejía
May 7, 2019·BioMed Research International·Fiordaliso C Román-CarraroSantiago Martínez-Calvillo
Mar 7, 2021·Genes·Luis E Florencio-MartínezSantiago Martínez-Calvillo

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