Abstract
Peripheral blood mononuclear cells (PBMCs) are stimulated by exercise and contribute not only to host defense, but also to growth, repair, and disease pathogenesis. Whether PBMC gene expression is altered by exercise in children is not known. Ten early pubertal boys (8-12 y) and 10 late pubertal boys (15-18 y) performed ten 2-min bouts of strenuous, constant work rate exercise with 1-min rest intervals. PBMCs were isolated before and after exercise and microarray (Affymetrix U133 + 2 chips) analyzed. Statistical criterion to identify gene expression changes was less than 5% false discovery rate (FDR) with 95% confidence interval. One thousand two hundred forty-six genes were altered in older boys (517 up, 729 down), but only 109 were altered in the younger group (79 up, 30 down). In older boys, 13 gene pathways (using Expression Analysis Systematic Explorer, p < 0.05) were found (e.g. natural killer cell cytotoxicity, apoptosis). Epiregulin gene expression (EREG, a growth factor involved in wound healing) increased in older boys. In older boys exercise altered genes such as TBX21, GZMA, PGTDR, and CCL5 also play roles in pediatric inflammatory diseases like asthma. Sixty-six genes were changed significantly in both groups. The ...Continue Reading
References
Mar 1, 1984·Journal of Applied Physiology: Respiratory, Environmental and Exercise Physiology·D M CooperK Wasserman
Jul 1, 1995·Medicine and Science in Sports and Exercise·R C BaileyD M Cooper
May 1, 1993·Journal of Applied Physiology·S ZanconatoD M Cooper
Nov 13, 2001·American Journal of Respiratory and Critical Care Medicine·P TirakitsoontornD M Cooper
Nov 13, 2002·Current Rheumatology Reports·Berent PrakkenSalvatore Albani
Aug 5, 2003·Journal of Cellular Biochemistry·Bradley K DraperLillian B Nanney
Jun 15, 2004·Journal of Applied Physiology·Peter H ConnollyDan M Cooper
Dec 8, 2004·Immunology and Allergy Clinics of North America·William W Storms
Dec 22, 2004·Pediatric Research·Jan J KaczorMark A Tarnopolsky
Apr 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·Kavitha Viswanathan, Firdaus S Dhabhar
Nov 29, 2005·The Medical Clinics of North America·John W Steinke, Larry Borish
Jan 10, 2006·BMC Bioinformatics·Pall F JonssonPaul A Bates
Mar 1, 2006·BMC Immunology·Rebecca LamasonKanneboyina Nagaraju
Aug 8, 2006·Nature Medicine·Jacob HannaOfer Mandelboim
Sep 28, 2006·Allergy·K BratkeJ C Virchow
Sep 28, 2006·Arthritis Research & Therapy·James N JarvisMichael Centola
Oct 6, 2006·British Journal of Sports Medicine·C D SchwindtD M Cooper
Oct 14, 2006·The Journal of Physiology·James G Tidball, Michelle Wehling-Henricks
Oct 14, 2006·Annals of the Rheumatic Diseases·F Hauet-BroereW Van Eden
Dec 21, 2006·Nature Immunology·Polly Matzinger
Jun 1, 2007·Genes and Immunity·G ZhuS G Pillai
Oct 24, 2007·The Journal of Allergy and Clinical Immunology·Monica Cheng Munthe-KaasDag Undlien
Nov 6, 2007·Frontiers in Bioscience : a Journal and Virtual Library·Martin Whitham, Matthew Benjamin Fortes
Nov 17, 2007·Journal of Applied Physiology·Shlomit Radom-AizikDan M Cooper
Nov 23, 2007·Biochemical Society Transactions·I Sabroe, M K B Whyte
Feb 2, 2008·Arthritis and Rheumatism·Elisabeth F ElstBerent J Prakken
Feb 16, 2008·Immunity·Douglas R Green
Feb 16, 2008·The Journal of Biological Chemistry·Kenneth LiuSteven Bozinovski
May 8, 2008·Physiological Genomics·Sashwati RoyChandan K Sen
Apr 1, 1988·Journal of Youth and Adolescence·A C PetersenA Boxer
Citations
Aug 8, 2013·Nucleic Acids Research·Gur YaariSteven H Kleinstein
May 2, 2009·Journal of Applied Physiology·Shlomit Radom-AizikDan M Cooper
Jan 30, 2010·Journal of Applied Physiology·Shlomit Radom-AizikDan M Cooper
Jun 28, 2011·BMC Bioinformatics·Christopher R BolenSteven H Kleinstein
Jan 7, 2014·PloS One·Stefano CapomaccioKatia Cappelli
Feb 22, 2011·Pharmacogenomics·Donna L Mendrick
Feb 12, 2014·Brain, Behavior, and Immunity·Asghar AbbasiHinnak Northoff
Oct 21, 2014·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·Qian KongJian-Pei Fang
Feb 28, 2015·Archives of Physiology and Biochemistry·Stine M UlvenKirsten B Holven
Jan 8, 2013·Annals of Human Genetics·Evadnie RampersaudPascal J Goldschmidt-Clermont
Mar 2, 2012·Clinical and Translational Science·Shlomit Radom-AizikDan M Cooper
Jan 18, 2011·Brain, Behavior, and Immunity·Gregory R AdamsDan M Cooper
May 26, 2015·Current Cardiovascular Risk Reports·Gyrd O GjevestadStine M Ulven
Dec 5, 2014·The British Journal of Nutrition·Tara Rendo-UrteagaAmelia Marti
Feb 13, 2018·Archives of Physiology and Biochemistry·Flávia Mariel StecklingFélix Alexandre Antunes Soares
Oct 28, 2019·Pediatric Research·Dan M Cooper, Shlomit Radom-Aizik
Aug 23, 2016·Aging·Elisa CalabriaCarlo Capelli
Aug 23, 2020·Head & Neck·Huixu XieLongjiang Li
May 31, 2014·Prague Medical Report·Magdalena C KimsaUrszula Mazurek
May 30, 2020·Cell·Kévin ContrepoisMichael P Snyder
Jan 5, 2021·European Journal of Clinical Investigation·Guilherme Eustáquio FurtadoAndré Luís Lacerda Bachi
Oct 23, 2020·Contemporary Clinical Trials Communications·R I LiemS Radom-Aizik