Abstract
Autoantibodies are clinically useful for phenotyping patients across the spectrum of autoimmune rheumatic diseases. Using serum from a patient with Sjögren's syndrome (SS), we detected a new specificity by immunoblotting. This study was undertaken to identify this autoantibody and to evaluate its disease specificity. A prominent 40-kd band was detected when immunoblotting was performed using SS patient serum and lysate from rat dorsal root ganglia (DRGs). Using 2-dimensional gel electrophoresis and liquid chromatography tandem mass spectrometry peptide sequencing, the autoantigen was identified as calponin 3. Anti-calponin 3 antibodies were evaluated in sera from patients with primary SS (n = 209), patients with systemic lupus erythematosus (SLE; n = 138), patients with myositis (n = 138), patients with multiple sclerosis (MS; n = 44), and healthy controls (n = 46) by enzyme-linked immunosorbent assay. Expression of calponin 3 was assessed by immunohistochemistry. Calponin 3 was identified as a new autoantigen. Anti-calponin 3 antibodies were detected in 23 (11.0%) of the 209 SS patients, 12 (8.7%) of the 138 SLE patients, 7 (5.1%) of the 138 myositis patients, 3 (6.8%) of the 44 MS patients, and 1 (2.2%) of the 46 healthy cont...Continue Reading
References
Mar 1, 1990·Annals of Neurology·J W GriffinE L Feldman
Dec 29, 1998·Archives of Neurology·J C McArthurJ W Griffin
Feb 27, 1999·Journal of Autoimmunity·M B FrankI N Targoff
May 15, 2002·Annals of the Rheumatic Diseases·C VitaliUNKNOWN European Study Group on Classification Criteria for Sjögren's Syndrome
Mar 18, 2005·The Journal of Pain : Official Journal of the American Pain Society·Michael I BennettJean Potter
Jun 15, 2007·Journal of the Peripheral Nervous System : JPNS·Weiran ChenAhmet Höke
Sep 10, 2010·The Neurologist·Julius Birnbaum
Mar 10, 2011·Annals of Neurology·Chris H PolmanJerry S Wolinsky
May 9, 2012·Arthritis Care & Research·S C ShiboskiUNKNOWN Sjögren's International Collaborative Clinical Alliance (SICCA) Research Groups
Aug 23, 2012·Journal of Immunological Methods·Tricia R CottrellLivia Casciola-Rosen
Aug 7, 2013·Glia·Li-Yen M HuangYong Chen
Aug 29, 2013·Medicine·Damien SèneJean-Pascal Lefaucheur
Feb 7, 2014·Oncoimmunology·Béatrice Sl PignoletRoland S Liblau
Mar 13, 2016·Gene·Rong Liu, J-P Jin
Apr 4, 2017·International Journal of Medical Sciences·Tim BothPaul L A van Daele
Citations
Nov 15, 2018·Modern Rheumatology·Toshio Odani, John A Chiorini
May 31, 2018·Nature Reviews. Rheumatology·Isobel Leake
Jun 18, 2019·Journal of Immunology Research·Yuebo JinZhanguo Li
Nov 7, 2019·International Journal of Molecular Sciences·Chiara RizzoGiuliana Guggino
Jul 24, 2020·Journal of Clinical Medicine·Dorian ParisisChristine Delporte
Feb 20, 2020·Der Internist·B Schlotter-Weigel
Dec 15, 2020·Journal of Autoimmunity·Bin WangGuixiu Shi
Jan 21, 2021·Journal of Clinical Medicine·Hirohito KotaniShinichi Sato