Brief Report: Point of Care Cryptococcal Antigen Screening: Pipetting Finger-Prick Blood Improves Performance of Immunomycologics Lateral Flow Assay

Journal of Acquired Immune Deficiency Syndromes : JAIDS
Rachel M WakeNelesh P Govender

Abstract

Cryptococcal antigen (CrAg) screening at the point of care could improve cryptococcal meningitis prevention where laboratory resources are limited. We evaluated the accuracy of Immunomycologics (IMMY, Norman, OK) CrAg lateral flow assay (LFA) using different techniques at point of care. Two tertiary-level hospitals in Johannesburg and a community health clinic in Soweto, South Africa. A case-control diagnostic validation study and a prospective clinic-based implementation study using the IMMY CrAg LFA on finger-prick blood. Accuracy, using direct application of LFA to sample, or pipette to transfer sample to diluent, and reading after 10 and 20 minutes, was compared with laboratory-based plasma testing. The validation study tested 64 CrAg-positive and 152 CrAg-negative patients with no symptoms or signs of meningitis, identified by routine laboratory screening, recruited by convenience sampling. Consecutively diagnosed HIV-infected adults (n = 654) were included in the implementation study. Sensitivity was 82% and 20% when the LFA was read 10 minutes after direct application to finger-prick blood in the validation and implementation studies, respectively. Using a pipette to transfer blood and reading after 20 minutes improved s...Continue Reading

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Oct 14, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rachel M WakeNelesh P Govender
Jan 26, 2018·Journal of Clinical Microbiology·Gar-Hing LeeMichael Leung

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Citations

Sep 28, 2018·Journal of Clinical Microbiology·Radha RajasinghamDavid R Boulware
Jun 23, 2020·Expert Review of Anti-infective Therapy·Greg GreeneJoseph N Jarvis
Dec 7, 2018·Current Opinion in Infectious Diseases·David S LawrenceJoseph N Jarvis
Jun 11, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rachel M WakeThomas S Harrison
Jan 10, 2021·Internal and Emergency Medicine·Kathryn Marie FisherBrit Long
Feb 19, 2021·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·Cornelia Lass-FlörlMiriam Knoll
Apr 23, 2021·Journal of Nanobiotechnology·Wei-Hsuan SungChao-Min Cheng

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