BrkAutoDisplay: functional display of multiple exogenous proteins on the surface of Escherichia coli by using BrkA autotransporter

Microbial Cell Factories
Fang SunFei Sun

Abstract

Bacterial surface display technique enables the exogenous proteins or polypeptides displayed on the bacterial surface, while maintaining their relatively independent spatial structures and biological activities. The technique makes recombinant bacteria possess the expectant functions, subsequently, directly used for many applications. Many proteins could be used to achieve bacterial surface display, among them, autotransporter, a member of the type V secretion system of gram-negative bacteria, has been extensively studied because of its modular structure and apparent simplicity. However, autotransporter has not been widely used at present due to lack of a convenient genetic vector system. With our recently characterized autotransporter BrkA (Bordetella serum-resistance killing protein A) from Bordetella pertussis, we are aiming to develop a new autotransporter-based surface display system for potential wide application. Here, we construct a bacterial surface display system named as BrkAutoDisplay, based on the structure of autotransporter BrkA. BrkAutoDisplay is a convenient system to host exogenous genes. In our test, this system is good to efficiently display various proteins on the outer membrane surface of Escherichia coli,...Continue Reading

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Citations

Mar 3, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Daehwan Kim, Seockmo Ku
Aug 6, 2021·Applied Biochemistry and Biotechnology·Lada E PetrovskayaMikhail P Kirpichnikov

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Methods Mentioned

BETA
surface display
fluorescence microscopy
flow cytometry
PCR
FACS

Software Mentioned

GeneWiz
AutoDisplay
BrkAutoDisplay

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