Broad and efficient control of major foodborne pathogenic strains of Escherichia coli by mixtures of plant-produced colicins

Proceedings of the National Academy of Sciences of the United States of America
Steve SchulzYuri Gleba

Abstract

Enterohemorrhagic Escherichia coli (EHEC) is one of the leading causes of bacterial enteric infections worldwide, causing ∼100,000 illnesses, 3,000 hospitalizations, and 90 deaths annually in the United States alone. These illnesses have been linked to consumption of contaminated animal products and vegetables. Currently, other than thermal inactivation, there are no effective methods to eliminate pathogenic bacteria in food. Colicins are nonantibiotic antimicrobial proteins, produced by E. coli strains that kill or inhibit the growth of other E. coli strains. Several colicins are highly effective against key EHEC strains. Here we demonstrate very high levels of colicin expression (up to 3 g/kg of fresh biomass) in tobacco and edible plants (spinach and leafy beets) at costs that will allow commercialization. Among the colicins examined, plant-expressed colicin M had the broadest antimicrobial activity against EHEC and complemented the potency of other colicins. A mixture of colicin M and colicin E7 showed very high activity against all major EHEC strains, as defined by the US Department of Agriculture/Food and Drug Administration. Treatments with low (less than 10 mg colicins per L) concentrations reduced the pathogenic bacter...Continue Reading

References

Jul 1, 1987·Journal of Bacteriology·J KöckV Braun
Nov 24, 2004·Journal of Food Protection·T R CallawayD J Nisbet
Sep 20, 2005·The Journal of Infectious Diseases·John T BrooksNancy A Strockbine
Sep 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Anatoli GiritchYuri Gleba
Oct 4, 2006·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T EiweggerZ Szépfalusi
Feb 28, 2009·Journal of Food Protection·Brenda S PattonJames S Dickson
Apr 1, 2009·Proceedings of the National Academy of Sciences of the United States of America·Melanie OeyRalph Bock
Nov 26, 2009·Molecular Nutrition & Food Research·Didier DupontAlan R Mackie
Mar 3, 2011·PloS One·Ernst WeberSylvestre Marillonnet
Jun 24, 2011·The New England Journal of Medicine·Christina FrankUNKNOWN HUS Investigation Team
Aug 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Stefan WernerYuri Gleba
Jul 3, 2013·International Journal of Environmental Research and Public Health·Claire VerraesLieve Herman
Jul 3, 2013·Bacteriophage·Olcay BoyaciogluIpek Goktepe
Jun 7, 2014·Frontiers in Microbiology·Shih-Chun YangJia-You Fang
Jul 1, 2014·BioMed Research International·Daniel TuséKaren A McDonald

❮ Previous
Next ❯

Citations

Sep 25, 2015·Proceedings of the National Academy of Sciences of the United States of America·Todd R Callaway, Trisha G Sheridan
Oct 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Paul WhiteColin Kleanthous
Jul 8, 2018·Applied and Environmental Microbiology·Maarten G K Ghequire, Başak Öztürk
Feb 1, 2019·Microbial Biotechnology·Maarten G K Ghequire, René De Mot
Mar 8, 2018·Scientific Reports·Tobias SchneiderYuri Gleba
Nov 11, 2019·Plant Biotechnology Journal·William M RooneyJoel J Milner
Oct 4, 2017·PloS One·Šarūnas PaškevičiusAušra Ražanskienė
Jul 29, 2020·Molecular Plant-microbe Interactions : MPMI·Erna DenkovskienėAušra Ražanskienė
Aug 29, 2020·Expert Review of Anti-infective Therapy·Juraj BosákDavid Šmajs
Mar 22, 2018·MBio·Maarten G K GhequireRené De Mot
May 17, 2018·Frontiers in Plant Science·Edoardo BertiniLinda Avesani
Apr 27, 2019·Frontiers in Plant Science·Simone Hahn-LöbmannYuri Gleba
Sep 15, 2020·Frontiers in Plant Science·Willian Souza Bernardes, Marcelo Menossi
Jul 14, 2018·Scientific Reports·Vaiva KazanavičiūtėAušra Ražanskienė
Oct 13, 2020·Frontiers in Microbiology·William M RooneyDaniel Walker
Jan 27, 2021·International Journal of Food Microbiology·Siyue XuVincent A Fischetti
Apr 21, 2017·Emerging Topics in Life Sciences·Hannah M BehrensColin Kleanthous
Nov 17, 2020·International Journal of Antimicrobial Agents·Hira MushtaqBashir Ahmad
Feb 3, 2021·Pest Management Science·Ray ChaiDaniel Walker
Mar 13, 2021·Foodborne Pathogens and Disease·Ruixia WangChao Shi
Jun 22, 2021·International Journal of Biological Macromolecules·Selvaraju KanagarajanLi-Hua Zhu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.