Broad connections in the Arabidopsis seed metabolic network revealed by metabolite profiling of an amino acid catabolism mutant

The Plant Journal : for Cell and Molecular Biology
Liping GuRobert L Last

Abstract

An Arabidopsis thaliana mutant was identified as having increases in 12 of 20 free proteogenic amino acids in seeds. Because these metabolites are produced from multiple, seemingly unrelated biosynthetic networks, it was not possible to use a candidate gene approach to discover the enzyme defect responsible for this complex syndrome. Complementary metabolite profiling analyses revealed increased seed homomethionine and isovaleroyloxypropyl-glucosinolate, along with reduced 3-benzoyloxypropyl-glucosinolate. These data led to the discovery of impaired branched chain amino acid catabolic enzyme isovaleryl-CoA dehydrogenase (encoded by gene At3g45300 or atIVD) as the cause of this metabolic syndrome. These results indicate that catabolism plays an important role in regulating levels of branched chain amino acids in seeds. The diverse set of metabolites affected in the ivd1 mutants suggests the existence of a more complex network regulating seed amino acid accumulation than previously observed. This combined targeted and non-targeted metabolite profiling approach is broadly applicable to the characterization of metabolic mutants, human disease studies and crop germplasm.

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Citations

Mar 26, 2011·Journal of Experimental Botany·Andrej Kochevenko, Alisdair R Fernie
Apr 21, 2012·Journal of Experimental Botany·Menny KirmaGad Galili
Aug 13, 2014·Journal of Experimental Botany·Réjane Pratelli, Guillaume Pilot
Mar 1, 2012·Current Opinion in Plant Biology·Mechthild Tegeder
Jun 21, 2011·Trends in Plant Science·Wagner L AraújoAlisdair R Fernie
Dec 28, 2010·Journal of Plant Physiology·Joost T van DongenAlisdair R Fernie
Feb 6, 2014·Rapid Communications in Mass Spectrometry : RCM·Jiangyin BaoA Daniel Jones
Feb 2, 2010·The Plant Journal : for Cell and Molecular Biology·Anthony SchilmillerRobert L Last
Sep 19, 2014·Frontiers in Plant Science·Gad GaliliAlisdair R Fernie
Jan 7, 2016·Annual Review of Plant Biology·Gad GaliliAlisdair R Fernie
Sep 20, 2015·Molecular Plant·Tatjana M HildebrandtHans-Peter Braun
Dec 10, 2016·PLoS Computational Biology·Sahra UygunShin-Han Shiu
Feb 1, 2017·The Plant Journal : for Cell and Molecular Biology·Fantao KongYonghua Li-Beisson
Mar 23, 2017·The Plant Journal : for Cell and Molecular Biology·Andrew B GipsonKerry A Rouhier
Jan 1, 2012·Metabolites·Eve Syrkin WurteleBasil J Nikolau
Sep 15, 2018·Journal of Experimental Botany·João Henrique F CavalcantiWagner L Araújo
Nov 9, 2018·Current Protocols in Plant Biology·Abou Yobi, Ruthie Angelovici
Oct 5, 2018·Frontiers in Plant Science·Christin LorenzTatjana M Hildebrandt
Jul 9, 2020·Frontiers in Plant Science·Qingqing YangQiaoquan Liu
Dec 26, 2013·The Plant Cell·Ruthie AngeloviciDean Dellapenna
Jan 21, 2014·The Plant Cell·Ronghui PanJianping Hu

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